amino acid/peptide/protein Flashcards
structure of a.a.
NH2 + COOH + H + R group
alpha carbon
carbon bonded to all groups
alpha prefix
extinguish alpha functional groups from side chains
zwitter ionic form
NH2 - gain H+ (cations) and COOH lose H+ (anion)
Acidic a.a function and example
more hydrophilic in protein - polar
aspartic and glutamic acid
side chain exposed to aq media
basic a.a.
lysine - pKa=9 - normal amine for protonation
arginine - pKa=14 - resonance stabilised guanidinium group - v. basic
histidine - pKa=6.8 - aromatic imidazole side chain - protonates = conjugate acid
functionalised a.a
H bonds of side chains when protein bind to molecules
Asparagine, Glutamine, Serine, Methionine, Threonine
Cysteine - form disulphide bond = rigid structure
hydrophobic a.a.
lipophilic side chains - bind for HP groups and recognition and binding event
Glycerine, Alanine, Valine, Leucine, Isoleucine, Proline
Aromatic a.a.
bulky and HP - interior of protein
Phenylalanine, Tryptophan - HP
Tyrosine - form H bonds
stereochemistry nomenclature
D- and L- - all alpha a.a in protein has L-
D- a.a.
found in peptidoglycan components of bacterial cell wall
D/L - rotatioin
different from R/S
uses CORN rule
CORN rule
- H placed below plane
- priority - C=O then R group then NH2
clockwise - CORN rule
D a.a.
anti-clockwise CORN rule
L a.a.