8.1 Flashcards
Griffith’s experiment
trying to develop a vaccine for pneumonia. R- strain not deadly S- strain deadly Heat killed s- strain- not deadly r starn plus heat killed s- strain deadly
This process was called transformation did not know that DNA was the transforming factor
Avery, Macleod, and McCarty
wanted to determine if DNA or proteins were the transforming factor. tested DNA, protein, enzymes, and RNA
Transformation cannot happen nwithout DNA
Hershey and Chase
Used T2 bacterophages which contain a protein shell and DNA on the inside. Vrisues infect bacteria and DNA infected bacteria came out not protein bacteria
Chargraffs rules
- # of guanine unites = cytosine, adenine = thymine
- amount o A,C,T,G varies for genetic diversity
Parts of a nucleotide
Phosphate, deoxyribose sugar, and a nitrogenous base
DNA strands are antiparallel
Complementary strands run in opposite directions
Phosphate- 5’
Hydroxyl- 3’
What bond holds nucleotides together
Hydrogen