8 Flashcards
Solvent extraction?
Fractionation due to differences in water solubility and entropy (hydrophilic and hydrophobic properties).
Is there somewhere were there isn’t volatile?
No, there’s volatile everywhere.
!!! Dynamic headspace collection flow?
!!!Different types of volatile extraction? 2•
•Dynamic headspace collection flow
•
Chromatography?
Separation of mixtures into their components by exploiting differences in chemical properties, such as size, charge, and polarity.
Stationary phase?
When chemicals don’t go anywhere, such as solvent.
Load sample?
Loads chemicals, sometimes via pipetting.
Elution via mobile phase?
When solvent moves down into a test tube by elutting. Eventually, different chemicals drip down at different rates, and we capture them in different test tubes.
Retention times?
How long it takes until a compound fully drips down.
Chromatography steps? 4•
•Stationary phase (solvent)
•Load sample (chemicals studied as one mixture)
•Elution via mobile phase to measure retention time of different compounds)
•Elution of fractionations
Elation of fractionations?
Now we can analyze the chemicals after fractionation.
!!!Is solvent the same thing as chemicals in stationary phase?
Thin layer chromatography (TLC)?
When you measure fractionation but instead of retention time, you move the stationary compound and then you look at the retention index that is the distanced measured by the origin and revealed by sample colour or indicator dye.
When doing chromatography, what questions do you need to ask? 3•
•What is the stationary phase
•What is the mobile phase
•What is the detector
If you integrate retention index in terms of time, what do you get?
How much of that compound is in there.
