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Molecular Cell Biology > 8 > Flashcards

8 Flashcards

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1
Q

Enzyme joining Okazaki fragments?

A

DNA ligase

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2
Q

Enzyme activity of topoisomerase II?

A

Reversible double stranded endonuclease

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3
Q

Enzyme inhibiting shortening of eukaryotic chromosomes?

A

Telomerase

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4
Q

Enzyme activity of telomerase?

A

Reverse transcriptase

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5
Q

Role of Telomerase in RNA?

A

Template for synthesis of telomere repeats

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6
Q

Consequence of decreasing proof reading activity during replication ?

A

Increasing rate of mutation

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7
Q

DNA damage caused by UV radiation?

A

Pyrimidine dimers

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8
Q

Repair system for replacement of damaged bases?

A

Base excision repair

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9
Q

Enzyme that can recognise and remove damaged base in base excision repair?

A

DNA glycosylase

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10
Q

Mechanism for removing pyrimidine dimers?

A

Nucleotide excision repair

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11
Q

Enzyme activity of exinuclease?

A

Endonuclease

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12
Q

Disease with defective nucleotide excision DNA repair?

A

Xeroderma pigmentosum

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13
Q

Repair mechanism responsible for correction of non-complementary base pairing?

A

Mismatch repair

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14
Q

Human disease with defective mismatch repair?

A

HNPCC (hereditary non-polyposis colon carcinoma)

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15
Q

What is translesion DNA synthesis?

A

Replication of damaged DNA

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16
Q

Main steps of transcription?

A

Initiation, elongation, termination

17
Q

Name of DNA region involved in initiation of transcription?

A

Promoter

18
Q

Initial and final sequences of transcription units?

A

Initial: promoter.
Final: terminator

19
Q

Name of transcription of step leading to the formation of phosphodiester bond?

A

Elongation

20
Q

Direction of RNA synthesis from POV of RNA?

A

5’—3’

21
Q

RNA produced by transcription, but not processed yet?

A

Primary transcript

22
Q

Components of RNA polymerase in bacteria?

A

Core enzyme and Sigma factor

Molecular Cell Biology (12 decks)

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