1

Question 1

Cellular organelles in eukaryotic cell only?

Answer 1

Nucleus, lysosome, mitochondria, centriole

Question 2

Method of introduction of foreign genes to DNA?

Answer 2

Transformation

Question 3

Gene transfer?

Answer 3

Prokaryotic- Transformation
Eukaryotic- Transfection

Question 4

Mechanism of penicillin?

Answer 4

Inhibits formation of bacterial cell wall

Question 5

Chromosome containing part of cell?

Answer 5

Nucleiod region

Question 6

Difference between DNA of prokaryotes and eukaryotes

Answer 6

Prokaryotes- shorter, circular, naked

Question 7

Viruses are obligate intercellular parasites?

Answer 7

Multiply only in infected cell

Question 8

Infections by bacteriophage?

Answer 8

Lysis and lysogenic

Question 9

Hypothesis describing origin of eukaryotic cells?

Answer 9

Endosymbiosis hypothesis

Question 10

Parameters affecting resolution power of LM?

Answer 10

Light wavelength
Refractive Index
Aperture angle of objective lens

Question 11

Magnifying component of LM?

Answer 11

Objective and ocular lens

Question 12

Calculate magnification of LM

Answer 12

Magnification of objective x ocular lens

Question 13

Image of LM?

Answer 13

Inverse and virtual

Question 14

Fixatives in microscopy?

Answer 14

Formaldehyde and osmium

Question 15

Technique to visualize protein?

Answer 15

Immunocytochemistry

Question 16

Relationship between accelerating voltage and wavelength?

Answer 16

Inverse

Question 17

EM method’s giving 3D?

Answer 17

Angular shadowing, Scanning Electron microscopy

Question 18

EM for studying isolation of organelles?

Answer 18

Angular and Rotary shadowing

Question 19

Techniques that use metal deposition on surface?

Answer 19

Angular and Rotary shadowing and SEM