7

Question 1

Protein phosphorylated by MPF?

Answer 1

Histone H1

Question 2

Main components of mitotic spindle?

Answer 2

Kinetochore(responsible for movement and separation of chromatids), polar(for elongation of cell), chromosomal and astral microtubules

Question 3

Protein complexes that hold sister chromatids?

Answer 3

Cohesin complex(cleaved by seperase)

Question 4

Phase of mitosis that chromatids separate?

Answer 4

Anaphase

Question 5

What is responsible for cytokinesis?

Answer 5

Actin filaments

Question 6

Cell division for germ cell?

Answer 6

Meiosis(n, haploid)

Question 7

Anaphase of 1st meiosis?

Answer 7

Homologous chromosomes separate

Question 8

Anaphase of 2nd meiosis?

Answer 8

Sister chromatids separate

Question 9

What is crossing over?

Answer 9

Exchange of genetic material between homologous chromosomes during meiosis

Question 10

Semi conservative nature of replication?

Answer 10

One chain of replicated DNA is the original and the other is the newly synthesised one

Question 11

Function of template during replication?

Answer 11

Helps incorporate nucleotide by base pairing

Question 12

Why is DNA replication primer dependent?

Answer 12

Because DNA polymerase are not able to initiate synthesis

Question 13

Continuously replicated strand of replication fork?

Answer 13

Leading strand

Question 14

Short DNA segments synthesised from lagging template strand of replication fork?

Answer 14

Okazaki fragments

Question 15

Site for begin of replication?

Answer 15

Origo (origin of replication)

Question 16

Enzyme causing DNA relaxation during replication?

Answer 16

Topoisomerase I

Question 17

Enzyme cleaving hydrogen bond at replication fork?

Answer 17

Helicase

Question 18

Function of primase?

Answer 18

Synthesises RNA primers

Question 19

Proofreading?

Answer 19

Removal of non complementary nucleotide incorporated during DNA replication

Question 20

Enzyme activity of polymerase I and III?

Answer 20

I: 5’—3’ elongation, 5’—3’ exonuclease
III: 5’—3’ elongation, 3’—5’ exonuclease

Question 21

Function of 3’—5’ exonuclease activity?

Answer 21

Proofreading

Question 22

Function of 5’—3’ exonuclease activity?

Answer 22

Primer degradation