2

Question 1

Recognition sequence of RE?

Answer 1

Palindromic sequence

Question 2

Example of palindromic with six basepairs?

Answer 2

GAATTC

Question 3

Types of enzymes that recognize palindromic?

Answer 3

RE, modification methylases

Question 4

DNA generated by RE?

Answer 4

Blunt ends and sticky end

Question 5

Methods that RE are paramount?;

Answer 5

Southern blotting,Cloning

Question 6

Vectors in molecular biology?

Answer 6

plasmid, cosmid

Question 7

DNA transcribed in host cell vector,

Answer 7

Experession vector

Question 8

Sequences isolated from only genomic library and not cDNA libraries?

Answer 8

Promoter, satellite DNA and intron

Question 9

Steps of PCR cycle?

Answer 9

Denaturation — primer binding — DNA synthesis

Question 10

Methods of amplifying DNA?

Answer 10

PCR and cloning

Question 11

PCR reaction to quantitate nucleus acid sequence ?

Answer 11

Real time PCR

Question 12

Molecular hybridization?

Answer 12

Renaturation of denatured nucleic acid with labeled probe

Question 13

Conclusion from southern blot?

Answer 13

Presence of gene and mutations
copy number of gene

Question 14

Method to visualize probe on southern blot?

Answer 14

Autoradiography

Question 15

Enzymes to prepare cDNA library?

Answer 15

DNA ligase and reverse transcriptase

Question 16

Example of biological gene transfer?

Answer 16

Physical- microinjection and gene gun
Chemical- transfection
Biological- virus vector

Question 17

Animals with artificially introduced gene in their genome?

Answer 17

Transgenic

Question 18

Phenomenon in KO mutation?

Answer 18

Homologous recombination

Question 19

Antisense oligonucleotide?

Answer 19

Oligonucleotide that can inhibit mRNA function by complementary base-pairing

Question 20

RNA molecules for RNA interference?

Answer 20

siRNA

Question 21

Physiological function of siRNA?

Answer 21

Regulation of gene expression

Question 22

Method for inhibition of proteins function?

Answer 22

Peptidomimetics, intracellular antibodies