3

Question 1

Tissue specific?

Answer 1

cDNA Library

Question 2

Methods for identifying specific proteins with specific antibodies?

Answer 2

Immunocytochemistry and western blotting

Question 3

Role of mRNA and oligo(dT) in in vitro cDNA system?

Answer 3

mRNA- template
oligo(dT)- primer

Question 4

Methods to prove actin gene expression in liver cells?

Answer 4

Northern and Western blotting

Question 5

Radioactive precursor to label products of PCR?

Answer 5

[3H] dTTP
[alpha- 32P] dCTP

Question 6

Difference between dTTP and ddTTP?

Answer 6

Absence of -OH group on 3’ position in ddTTP

Question 7

Use of SNP chips?

Answer 7

To identify many single nucleotide polymorphisms units DNA samples

Question 8

What is GMO?

Answer 8

Genetically modified organisms

Question 9

Use of cDNA chips?

Answer 9

To analyze expression of many genes in a cell

Question 10

Visualize result of molecular hybridization?

Answer 10

Autoradiography

Question 11

Subcellular fractions and order of generation?

Answer 11

Nuclear — mitochondrial — microsomal — ribosomal — cytosol

Question 12

Cellular organisms in microsomal?

Answer 12

Golgi, RER, SER

Question 13

Separation methods for 2D electrophoresis of proteins?

Answer 13

Isoelectric focussing and SDS gel electrophoresis

Question 14

Charge of proteins in solution with pH lower than isoelectric point of the protein?

Answer 14

+

Question 15

Charge of proteins at the end of isoelectric focussing?

Answer 15

No charge

Question 16

Charge of proteins at the end of SDS Page?

Answer 16

-

Question 17

Electrophoretic method for protein separation based on mass?

Answer 17

SDS page

Question 18

Methods independent of mass

Answer 18

Affinity chromatography
Isopycnic density gradient centrifugation

Question 19

Relationship between elution volume and mass?

Answer 19

Higher elution volume = lower mass

Question 20

Ligands used to separate during affinity chromatography and what they separate?

Answer 20

Antigen — antibody
Hormone — receptor

Question 21

Separation of poly(A)+mRNA from RNA fraction?

Answer 21

Oligo(dT) affinity chromatography

Question 22

Type of electrophoresis in DNA sequencing?

Answer 22

Capillary electrophoresis