7.9 Investigating transport in plants Flashcards

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1
Q

Ways of investigating translocation in plants

A
  • ringing experiments
  • tracer experiments
  • aphids
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2
Q

How do ringing experiments work?

A
  • cut protective layer and phloem around woody stem
  • stem immediately above will swell
  • non-photosynthetic tissues bellow wither and die
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3
Q

What is the substance found above the ring rich in?

A
  • sugars
  • dissolved organic substances
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4
Q

What does the ringing experiment show?

A
  • interruption in the flow of sugars below the ring
  • phloem and not xylem is responsible for translocating years
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5
Q

How does the tracer experiment work?

A
  • cut thin cross sections of plant grown in 14CO2
  • place on x-ray film

Blackened film = 14C
No Blackened film = no 14C

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6
Q

What is the explanation for the type of plant used in tracer experiments?

A

Isotope 14C is used to make 14CO2

Plants grown in 14CO2 have 14C incorporated in the sugars

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7
Q

What does the tracer experiment show?

A
  • blackened tissue is phloem
  • only phloem carries sugars
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8
Q

How are aphids used to investigate transport in plants?

A
  • needle like mouthparts
  • penetrate the phloem
  • extract contents from sieve tube
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9
Q

What do aphids show?

A
  • shows daily variation in the sucrose contents of leaves
  • mirrored in the phloem
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