Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

bio > 6.1.1 cellular control > Flashcards

6.1.1 cellular control Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

mutation

A

random change in base sequence of DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what causes a mumtation

A

mutagen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

examples of mutagens

A
  • tar in tobacco smoke
  • ionising radiation
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

2 classes of mutation

A
  • point mutation
  • insertion/deletion (indel)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

point/substitution mutation

A

one base pair replaces another

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

indel mutation

A
  • one or MORE nucleotides are INSERTED or DELETED from a legnth of DNA
  • can cause a frameshift
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

3 types of point mutation

A
  1. silent
  2. missense
  3. nonsense
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

silent mutation

A

triplet code is changed but due to the degenerate nature of code the same amino acid is still produced
- primary secondary tertairy structure of protein all the same

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

inversion mutation

A
  • eg tac becomes cat
  • affects one amino acid
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

missense mutation

A
  • change in base triplet resulting in a different amino acid produced
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

nonsense mutation

A
  • base code becomes a STOP triplet
  • truncated protein that wont function
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

frameshift

A
  • caused by INDEL mutation
  • when base pairs are inserted/deleted not in threes, due to the code being NON OVERLAPPING
  • all the subseqeuent base triplets are altered; all amino acids are altered
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

expanding triple nucleotide repeat

A
  • repeating triplet sequence repeats even more
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

why could silent be harmful?

A
  • could code for RNA that regulates the expression of other genes
  • could incorrectly regulate this
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

exon

A

coding region of DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

intron

A

non coding region of DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

operon

A

a group of genes that function as a single transcription unit

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

transcription factors (eukaryote)

A
  • proteins that act to control which genes are turned ON or OFF
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

simplified, how do TF work

A

ACTIVATOR:
- bind to promoter region of gene, aiding RNA polyemerase to bind to transcribe the gene
REPRESSOR:
- binds to gene, BLOCKS rna polymerase from binding

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

operon

A

a section of DNA containing a cluster of genes which are controlled by a single promoter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

3 parts of the operon (link to lac)

A
  1. structural genes: (lac z,y,a)
  2. control elements: (promoter for RNA p to bind and operator where transcription factors can bind)
  3. regulatory gene: (lac I) codes for transcription factors
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

ecoli relation to lactose

A
  • e coli normally respires glucose
  • if glucose is absent, and LACTOSE present, it induces the production of:
    1. lactose permease
    2. beta galactosidase
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

importance of the fact that e coli can only produce these enzymes when glucose absent and lactose present?

A
  • doesnt waste ATP and amino acids on making proteins they dont need
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

what codes for lactose permease

A

lac Y

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

what codes for beta galactosidase

A

lacZ

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

beta-galactosidase purpose

A

breaks glycosidic bonds; hydrolyses lactose to glucose and galactose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

what actually induces the enzymes

A

LACTOSE (but absence of glucose is needed)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

what is the purpose of the lac operon

A

contains the genes that produce the enzymes needed to respire lactose: lactose permease and beta galactosidase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

lac operon structure

A

DRAW
IPOZYA
1. lac I
2. promoter
3. operator
4. lac z
5. lac y
6. lac a

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

structural genes

A

genes that code for proteins NOT INVOLVED in DNA regulation
lac z,y,a

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

are lac z, y , a involved in DNA regulation ?

A

NO

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

lac I

A
  • regulatory gene (codes for proteins involved in DNA regulation)
  • codes for a REPRESSOR PROTEIN
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

operator (lac opeoron)

A

DNA sequence to which repressor protein binds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

promoter (lac operon)

A

DNA sequence to which RNA polymerase binds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

homebox sequence

A

REGULATORY GENE THAT CONTROLS BODY DEVELOPMENT
- contains 180 base pairs, encoding a 60 amino acid HOMEODOMAIN seqeucne that is part of a protein
- control body development

36
Q

what does the homeodomain sequence do

A
  • fold into a particular shape and bind to DNA
  • act as TRANSCRIPTION FACTORS, switching some genes on and repressing others
37
Q

homeobox genes highly conserved ; what does that mean

A
  • remained in all descendent species throughout evolutionary history
  • little difference between the hbox genes of diff species
38
Q

HOX genes

A
  • subset of homeobox genes, ONLY FOUND IN ANIMALS, involved in formation og anatomical features in correcet locations of body plan
39
Q

where are homeobox genes found

A

animals, plants, fungi

40
Q

regulatory genes

A

genes that code for proteins that are involved in DNA repllication

41
Q

what do homeobox genes also regulate …

A

-mitosis and apoptosis (responding to internal and external stimuli)

42
Q

apoptosis defintion

A

programmed cell death

43
Q

steps of apoptosis

A
  1. enzymes break down the cell cytoskeleton
  2. cytoplasm becomes dense with tightly packed organelles
  3. cell surface membrane changes; small protrusions called BLEBS form
  4. chromatin condenses, the nuclear envelope breaks and DNA breaks into fragments
  5. the cell breaks into vesiclaes that are ingested by phagocytes
44
Q

what can apoptosis + mitosis be triggered by

A

internal and external stimuli, to control rate of growth

45
Q

apoptosis and mitosis relation

A
  • rate of cell death should = reate of cells produced
    1. not enough apoptosis = tumours
    2. too much apoptosis = cell loss and degeneration
46
Q

POST TRANSCRIPTIONAL GENE EXPRESSION in one line

A

modifying mRNA so it can be translated

47
Q

primary-mRNA

A
  • contains a mix of introns and extrons; freshly transcribed from the DNA
48
Q

what enzyme is used in splicing

A

endonuclease

49
Q

translational level of gene expression

A
  • regulation of translation (if it happens or no)
50
Q

lac operon: normal circumstances, glucose present

A
  1. regulatory gene Lac I codes for REPRESSOR protein.
  2. this TRANSCRIPTION FACTOR binds to the OPERATOR
  3. repressor TF is large so BLOCKS the promoter from being bounded to the RNA polymerase
  4. therefore transcription of the structural genes (z,y,a) can’t occur as ur respiring glucose instead
51
Q

lac operon: glucose NOT present

A
  1. regulatory gene lac I codes for REPRESSOR protein
  2. LACTOSE binds to the transcription factor REPRESSOR PROTEIN, causing a confirmational change of the REPRESSOR.
  3. Repressore therefore cannot bind to the operator
  4. promoter is free, RNA polymerase binds, lac zya STRUCTURAL PROTEINS are coded for
  5. beta galactosidase and lactose permease enzymes are coded for and lactose can be respired
52
Q

what DNA contains introns

A

ONLY EUKARYOTES, not prokaryotes

53
Q

splicing; post transcriptional control

A
  • primary mRNA contains introns and extrons
  • need to be MODIFIED to REMOVE introns and JOIN exons
  • produces mature mRNA ready for translation
54
Q

example of post translational gene regulation

A
  • activation of proteins
  • eg phosphorylation of enzymes BY CAMP
55
Q

How does cAMP work in post translational gene regulation

A
  1. HORMONE, binds to receptor on CSM of the target cell
  2. confrimational change in shape of receptor, which activates a G protein
  3. G protein activates ADENYL CYCLASE ENZYMES
  4. CATALYSES conversion of ATP to cAMP
  5. cAMP binds to PKA to activates
  6. Activated PKA catalyses the phosphorylation of various proteins (so enzymes are activated), hydrolysing ATP in the process.
  7. PKA can also phosphorylate another protein wchich can enter the nucleus and act as a transcription factor
56
Q

how to homebox genees actually work

A
  • homedomain sequence
  • codes for TRANSCRIPTION FACTORS that can activate or suppress gene expression WHEN NEEDED during development of a zygote
57
Q

DNA, mRNA, tRNA relations

A

DNA=> mRNA is complementary but U instead of T => tRNA is complementary (and same as DNA but U instead of T)

58
Q

is meiosis involved in body plan development

A

NO

59
Q

cell cycle order

A

G1
S
G2
Mitosis (PMAT)
Cytokinesis

60
Q

why are genes not expressed during cell division

A

HETEROCHROMATIN
- chromasomes are vv tightly wound around histones

61
Q

euchromatin

A

chromosomes wound less tighlty around histones; genes can be expressed

62
Q

explain how a mutation could have no effect on function of protein (4)

A
  1. degenrate triplet codes
  2. same amino acid seqeucne
  3. chemically simialr AA produced; doenst alter shape of protein
  4. mutation in an intron
63
Q

explain how a mutation could have an affect on function of protein(4)

A
  1. indel mutation = frame shift
  2. all codons after affected
  3. different sequence of amino acids
  4. different tertiary structure
64
Q

explain IN DETAIL (5) the change in triplet on change in primary structure; LINKING TO TRANSLATION

A
  1. diff base sequence of DNA
  2. diff mRNA triplet
  3. diff tRNA ANTICODN
  4. tRNA brings a different amino acid
  5. different primary structure of amino acids
65
Q

why light microscope (4)

A
  1. mag within range
  2. smaller organelles not visible
  3. colour
  4. wide field of view
66
Q

uses of mitosis (4)

A
  1. growth and repari of tissues
  2. body plan
  3. clinal expansion
  4. asexual reproduction
67
Q

explain how transcription factors work (4)

A
  1. hormone enters cell
  2. binds with the transcription factor to activate it
  3. TF binds to promoter
  4. allows RNA polymerase to bind, genes can be expressed
68
Q

describe post transcriptional regulation (5)

A
  1. primary mRNA modified
  2. removal of introns and joining of exons
  3. ALTERNATIVE SPLICING CAN PRODUCE VERSIONS OF MRNA THAT CODES FOR ANOTHER PROTEIN
  4. cAMP phosphorylates (activates) enzyme
  5. binding may alter shape
69
Q

ROLE OF camp in post translational regulation (2)

A
  1. activates PKA which activates the protein by phosphorylation
  2. the biding may alter the shape of the protein
70
Q

what makes galactose a good respiratory substance (3)

A
  1. polar OH groups = soluble
  2. soluble so can move/be transported
  3. bonds contain energy; can be broken
71
Q

why does lactose need lactose permease? (2)

A
  • too large
  • to fit between phospholipids
72
Q

why use x animal for investigations in body plan? (2)

A
  • cheap
  • rapid reproduction
73
Q

examples of molecules that signal apoptosis (3)

A
  • cytokines
  • hormones
  • nitric oxide
74
Q

describe how failure of hox gene means fingers stick together? (3)

A
  • correct transcription factor is not produced
  • molecules signalling apoptosis (hormones etc) not produced
  • apoptosis not induced
75
Q

Explain why some regions of DNA can be described as ‘non-coding’ (4)

A
  • mRNA modified
  • splicing; introns removed so aren’t in mature mRNA
  • are not translated
76
Q

why is theremuch more variation in non-coding DNA?

A

not selected against; doesn’t affect survival

77
Q

is cAMP a protein

A

no

78
Q

RNA vs DNA polyemrase (4)

A

RNA
- used in transcription
- to produce mrNA
- one strand formed
DNA
- used in DNA replication
- 2 strands formed
- before mitosis

79
Q

state what is meant by a homeobox gene (2)

A
  • regulatory gene
  • 180 base pairs ( codes for homeobox sequence)
  • codes for TRANSCRIPTION FACTORS that control anatomical body plan
80
Q

why are homeobox genes highly conserved (4)

A
  1. they are extremely important
  2. would alter body plan
  3. would affect many other genes
  4. likely to have LETHAL effects so be selected against
81
Q

why are stop codons described as such

A

don’t code for an amino acid

82
Q

why is yogurt containing bacteria safe for lactose intolerant people to eat? (2)

A
  • bacteria respire the lactose anaerbocially
  • produce lactic acid
83
Q

first thing homebox genes do

A

head tail orientation

84
Q

difference between apoptosis and necrosis (3)

A
  • apoptosis is active programmed cell death
  • necrosis is passive cell death caused by environmental factors ; trauma
  • a = regulated, n= unregulated
  • a = no inflammation or damage to surrounding tissues , n = yes damage
85
Q

how does mutation lead to a truncated protein ? (4)

A
  • nonsense point mutation
  • base changed, stop codon; no amino acid coded for
  • translation termianted
  • no aa added thereafter
86
Q

why is the genetic code described as universal?

A
  • same 4 nitrogenous DNA bases (a,t,c,g) in all species
  • same triplet codes for same amino acid in all species

bio (16 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions