604-700 Flashcards
1
Q
- K39 and FAST (Fast Agglutination Screening Test) for Leishmania
- Rupture of RBCs every 72 hours: P. MALARIAE
- Band trophozoite: P. MALARIAE
- Amoeboid trophozoite: P. VIVAX
- Crescent-shaped gametocytes: P. FALCIPARUM
A
- Cyclospora cayetanensis: cyanobacterium-like body (CLB)
- Circumoval Precipitin Test (COPT) SCHISTOSOMES
- Sabin Feldman Dye test: TOXOPLASMA
- Sheather sugar flotation technique: CRYPTOSPORIDIUM
2
Q
- Stool preservatives: formalin, Schaudinn’s solution, PVA (polyvinyl alcohol), MIF (merthiolate-iodine-formalin) and SAF (sodium acetate-acetic acid formalin)
A
- Fecal concentration procedure (formalin-ether/ethyl acetate) FOUR LAYERS a) top layer of ether or ethyl acetate, b) a plug of fatty debris adherent to the wall of the tube, c) layer of formalin, and d) sediment.
3
Q
- DELAFIELD HEMATOXYLIN stain is mainly useful in demonstrating the detailed structures of MICROFILARIAE.
A
- Iron conversion factor from conventional to SI (µmol/L): 0.179
- Bilirubin conversion factor from conventional to SI (µmol/L): 17.1
- Thyroxine conversion factor to SI (µg/dl to nmol/L): 12.9
4
Q
- Specimen collection & processing: Pre-analytical QA
- Long-term accuracy of analytical methods: External QC
A
- Abrupt change: Shift
- Gradual change: Trend
- One control value exceeds +2s and another exceeds -2s: R4s
- 2 consecutive ctrl values exceed the same mean +2s or -2s: 2:2s
5
Q
- Fixed-angle centrifuge advantages over the horizontal centrifuge: Lesser air friction, smaller increase in sample temperature, quicker sedimentation of small particles, and operated over higher speed
A
- Used to determine whether there is statistically significant difference between the SD of 2 groups of data: f-test
- Used to determine whether there is statistically significant difference between the means of 2 groups of data: t-test
6
Q
- Sample of known quantity with several analytes.: Control
- Anticoagulant for cardiopulmonary bypass: Heparin
- Basal state collection: Early morning blood collection
A
- Uses 2 monochromators, affected by quenching: Fluorometry
- Uses 2 photodetectors, for the sample beam and reference beam: Double – beam in space
7
Q
- Obsolete blood glucose methodologies: Folin- Wu, Nelson Somogyi
- Chemical method for glucose, still widely used: Ortho-toluidine, condensation method
- Test for chylomicrons, creamy layer on top: Standing plasma test
A
- Apolipoprotein component of VLDL: Apo-B100
- Transports exogenous triglycerides: Chylomicrons
- Transports endogenous triglycerides: VLDL
- Highest cholesterol content: LDL
- One step method for cholesterol determination: Liebermann - Burchardt
8
Q
- Cholesterol esterase: Used in enzymatic method of cholesterol determination
- CV of HDLc (NCEP Guidelines for Acceptable Measurement Error): ≤ 4%
- Assay for Uric acid that uses mercury arc vapor lamp: Enzymatic: UV
A
- Greater specificity and more expensive BUN assay: Enzymatic: ammonia formation
- Simple, Nonspecific test for Creatinine determination: Colorimetric: end point
9
Q
- Categories of Azotemia: Pre-renal, Renal, Post-renal
- Test used to assess the ability to conjugate bilirubin and secrete bile: SERUM BILIRUBIN LEVELS
A
- Gamma spike/ Monoclonal gammopathy: Multiple myeloma
- Beta-gamma bridging: Hepatic cirrhosis
10
Q
- Reaction rate is directly proportional to substrate concentration: First-order kinetics
- Enzyme specific for both pancreas and salivary glands: Amylase
- Clinically significant if decreased: Cholinesterase
A
- Substrate used in Bowers-McComb method for ALP activity measurement: p-nitrophenylphosphate
- Chief counterion of sodium: Chloride
- Driving force of bicarbonate buffer system: CARBON DIOXIDE
11
Q
- Chloride and Bicarbonate relationship: Reciprocal
- Confirmatory test for Acromegaly: Glucose suppression test
A
- Increased in Hypothyroidism (primary): TSH
- T3 uptake levels in Hypothyroidism : Decreased
- Begins with patient identification and continues until testing is completed and the results are reported : Chain of custody
12
Q
- Requires whole blood EDTA sample: Cyclosporine and Tacrolimus tests
- Method of choice for measuring antidepressants: HPLC
- Gold standard for drug testing: GC-MS
- Inhibits acetylcholinesterase: Organophosphates & Carbamates
A
- Dissociable substance that yields hydrogen ions: Acid
- Dissociable substance that yields hydroxyl ions: Base
- Dissociable substance that accepts hydrogen ions: Base
- Dissociable substance that accepts hydroxyl ions: Acid
13
Q
- Comparing patient’s results with previous results: Delta check
- POCT is most often performed by nurses, perfusionists (who operate heart-lung machine during open heart surgery), respiratory therapists and physician themselves.
A
- Expressed in Ehrlich units (mg/dl): Urobilinogen
- Used to differentiate myoglobin and hemoglobin: Blondheim’s test
14
Q
- Degree of Hazard 1: Slight hazard
- Degree of Hazard 2: Moderate hazard
- Degree of Hazard 3: Serious hazard
- Degree of Hazard 4: Extreme hazard
A
- Handwashing: Clean between fingers 15 sec (or 20 sec , 6th ed), downward
- When skin or eye contact occurs, the best first aid is to immediately: Flush the area with water for at least 15 minutes and then seek medical attention
15
Q
- Preservative of choice for urine cytology studies: Saccomano’s fixative
- Urine specimen for Urobilinogen: Afternoon sample (2-4pm)
- Storage conditions for reagent strip: Cool, dry place
A
- Reagent incorporated in Ketone pad: Sodium nitroprusside
- Hoesch Test: Ehrlich’s reagent in 6M HCl
- Speckled pattern on blood parameter: Hematuria
