5.2 Enzymes and Metabolic Pathways Flashcards
What is a metabolic pathway?
a series of linked reactions that begin with a particular reactant and terminate with an end product
Some metabolic pathways are cyclical. What does cyclical mean?
regenerating the starting material
When is energy captured and used more easily?
if it is released in small increments rather than all at once
What are enzymes?
typically proteins that function as catalysts to speed a chemical reaction
What are ribozymes?
a form of RNA molecules that can act as catalysts
What do catalysts do?
participate in chemical reactions, but are not used by the reaction
What determines whether a reaction goes forward?
NOT THE ENZYME
the free energy of the reaction
What is a substrate?
the reactants in an enzymatic reaction for that enzyme
What does the presence or absence of an active enzyme determine?
which reaction takes place
How is activation achieved in the absence of an enzyme?
by heating a reaction flask to increase the number of effective collisions between molecules
What is energy of activation?
energy that must be added to cause molecules to react with one another
What needs to happen in order for a reaction to occur?
even though the reaction will proceed (free energy is negative), energy of activation must be overcome
What kind of reaction is the burning of firewood?
very exergonic reaction, but firewood in a pile does not spontaneously combust
What is required to overcome the energy of activation?
the input of some energy
What do enzymes do to energy?
enzymes lower the amount of energy required for activation to occur
What does the addition of an enzyme change?
NOTHING, it does not change the end result of the reaction
What results in a negative amount of free energy?
energy of the products is less than then energy of the reactants, but the reaction will not go at all unless the energy of activation is overcome
What happens when you lower the energy of activation?
enzyme increases the rate of reaction
Why will sugar in your kitchen breakdown into CO2 and H2O?
because the energy of the products is much less than the free energy of the reactant, the reaction is really slow
What happens when you eat the sugar?
enzymes in your digestive system greatly increase the speed at which the sugar is broken down, the end result is still the same
What is the active site?
one small party of the enzyme that, in most instances, complexes with the substrate
What happens at the active site?
enzyme and substrate fit together and form an enzyme-substrate complex
What is the induced fit model?
the active site undergoes a slight change in shape in order to accommodate substrates
the enzyme is induced to undergo a slight alteration to achieve optimum fit