4.5 DNA Cloning Flashcards
What has our knowledge of DNA biology led to?
an ability to manipulate the genes of organisms
What is a genome?
the complete genetic makeup of an organism
What is genetic engineering?
practice of cloning genes and then using them to alter the genome of viruses and cells
What are the uses of genetic engineering?
ranges from producing a product to treating genetic disorders
What is cloning?
the production of identical copies of an organism through asexual means
Why are human identical twins also considered clones?
because the first two cells of the embryo separated and each became a complete individual
What is gene cloning?
the production of many identical copies of a single gene
Why do biologists clone genes?
- want to produce large quantities of the gene’s protein product such as human insulin
- learn how a cloned gene codes for a particular protein
- use the genes to alter the phenotypes of other organisms in a beneficial way
What is gene therapy?
when cloned genes are used to modify a human
What are transgenic organisms?
organisms with foreign DNA or genes inserted in them
What are transgenic organisms frequently used for?
to produce a product desired by humans
What do most processes rely on to produce cloned DNA?
recombinant DNA technology and the polymerase chain reaction
What is recombinant DNA (rDNA)?
contains DNA from 2 or more different sources such as the human cell and the bacterial cell
What do researchers need to make rDNA?
vector
What is a vector?
a piece of DNA that can be manipulated such that foreign DNA can be added to it