5.1 DNA Flashcards
What disease was Griffith originally researching?
pneumonia
What injection experiments did the mice die from?
injected with both rough strain and heat killed smooth strain together and smooth strain
What injection experiments did the mice live from?
injected with rough strain and heat killed smooth strain
How can good bacteria’s become bad bacteria?
transformation
Why did the rough strain and heat killed smooth strain become deadly once combined?
the living bacteria stole some kind of molecule from the dead bacteria, leading to transformation
What possible bacteria’s could the living bacteria have stolen from the dead?
DNA, protein, lipid, proteins, ect.
What was the transforming factor in Griffith’s experiment?
DNA
Why did Avery, MacLeod, and McCarty want to find the transforming factor in Griffith’s experiment?
they wanted to determine if protein or DNA was the transforming factor and therefore responsible for heredity
Why does a virus infect genetic material into a host cell?
so they can spread/reproduce
What is phase 1 in the Hershey and Chase experiment?
one batch of viruses was labeled with radioactive phosphorus which is incorporated in the DNA, another batch of viruses was labeled with radioactive sulfur, which is incorporated into the protein coat
What is phase 2 in the Hershey and Chase experiment?
bacteria were infected with the viruses, the researchers were looking to identify if viral DNA or viral protein entered the host bacteria cell
What is phase 3 in the Hershey and Chase experiment?
the cultures were blended and centrifuged to separate the viruses from the bacteria
What is phase 4 in the Hershey and Chase experiment?
bacteria infected with the DNA marked viruses contained phosphorus and the bacteria infected with the protein marked viruses contained no markers
What are Charagaff’s rules?
A=T and G=C (the base pairs)
What are the parts of a nucleotide?
phosphate, sugar, and a nitrogen base
