5-6 Microscope Flashcards
Artisole made careful observations and descriptions of…
More than 500 animal species and set up a classification system based on observations.
Hans and Zacharias Janssen…
Invented the microscope in 1595.
Hans and Zacharias used…
Ocular and Objective lenses
Robert Hooke was using a…
Hand microscope that had three lens system
The illumination was a beam of light concentrated on a specimen by…
Passing light through a water filled glass.
Robert Hooke published his Micrographia containing…
38 illustrations of plant, animal, and non-living objects viewed through a microscope
Hooke discovered that the cork primarily of…
Air pockets surrounded by a thin mesh of fibre.
Antoni Van Leeuwenhoek, using only a simple…
Single lens microscope rather like a magnifying glass.
Van Leeuwenhoek’s drawings were called…
Animacules
Van Leeuwenhoek’s microscope was…
Held directly at the eye
His microscopes had a smaller lens which…
Provided higher magnification
Early compound microscopes…
Were less efficient
Field of view:
The area you see through the eye piece of a microscope.
Francesco Redi questioned…
The belief that maggots appeared spontaneously from raw meat
John Needham proved that living things…
Could be produced from non-living matter
Lazzaro Spallanzani proposed…
That there were responsible for the new growth
Life Forces:
A natural force postulated to produce life spontaneous.
Critics suggested that all Spallanzani has shown was…
That air was required for spontaneous generation to occur
Louis Pastuer submitted the experiment of…
Boiling meat broth in a flask
Robert Brown identified an important…
Cell structure, the nucleas.
Nucleas: (in cells)
Organelle that contains DNA, genetic material, and directs all cellular activities
M.J. Scgleider observed that all plants were composed of cells and he proposed…
That the nucleas was the structure responsible for the development of the cell.
Theodor Schwann believed that there must be…
Similarities between plant and animal tissue
Brown and Schlelden proposed that all plants and animal were composed of…
Cells and that the cell was the basic unit of all organisms.
All living things are made up of…
One or more cells and the materials produced by these cells
All life functions take place in cells…
Making them the smallest units of life
All cells are produced from pre-existing cells…
Through the process of cell division
Light microscopes magnify cells through the use of…
One or more curved lenses and a light source,
Compound light microscopes is an…
Important magnifying tool
Research light microscopes have a maximum magnification of…
1000-2000x
When light passes directly through cells its called a…
Bright field
Brightfield:
Illumination in the light microscopes in which the specimen is illuminated by a unfiltered beam of white light that passes from the illumination source through the specimen into the objective, and then to the eye piece
Scientists discovered that manipulating the light source could…
Alter the contrast between structures in the cell and improve the image
Resolution or resolving power:
The ability to distinguish between two structures that are close together
A microscope must provide the…
Capability to see smaller stuff
The efficiency of light microscopes is limited because…
As light is focused into smaller and smaller diameters the image becomes blurred
Fluorescence microscopy was invented to give information about…
Molecules on the cell service
The fluorescent molecule emitted…
Lights of different wave lengths
J.H Coons used anitbody molecules labelled with a fluorescent substance…
To show antigens were on the surface of cells
Confocal Microscope:
A microscope that uses confocal technology
Laser concentrates light onto a…
Specimen
The reflection is passed through a tiny opening called the…
Confocal pinhole and reaches an electronic detector that converts the light into an image
The process called dendrites that bring information to the…
Cell body of the motor neuron merely appear fibrous, but, using confocal technology
James Hillier and Albert Prebus developed…
The first functional electron microscope
Electron Microscope:
A microscope that uses a beam of electrons to produce images of fine detail
The image is formed by the absorption or scattering of the electron beam because…
Electron dense materials do not let the electrons pass through.
Electron-dense:
Characteristic of a substance that does not allow electrons to pass through it, but either absorbs or scatters the electrons
Transmission Electron Microscope:
Depends on a beam of electrons passed through a very thin section of fixed and stained tissue embedded in plastic
Scanning Electron Microscope:
Gives information about the surface features a specimen
Specimens are fixed and covered with an…
Electron-dense material like gold, which reflects specimen
One of the drawbacks of TEM is…
building a three dimensional object
Total =
Ocular x Objective
