5-28

Question 1

what is the motility test medium composed of?

Answer 1

semi solid medium with 0.4% agar concentration

2,3,5-Triphenyltetrazolium chloride

Question 2

what are the color of 2,3,5-Triphenyltetrazolium when oxidized and reduced?

Answer 2

2,3,5-Triphenyltetrazolium is

colorless when oxidized

red when reduced

Question 3

how will a motile bacterium be indicated in a motility test?

Answer 3

radiate away from stab line (+)
(converting oxidized 2,3,5-Triphenyltetrazolium chloride to a red reduced 2,3,5-Triphenyltetrazolium)

stay at stab line (-)

Question 4

what does a motility test indicate?

Answer 4

flagella is preset

Question 5

why do you need central stab line?

Answer 5

determine results whether positive or negative, otherwise results are obscured

Question 6

why is 0.4% agar used?

Answer 6

to allow bacteria to move through medium