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Micro prac 1 > 1-2 > Flashcards

1-2 Flashcards

1
Q

what does a media/medium contain?

A

carbon sources
nitrogen
vitamins
minerals

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2
Q

Microbiological media usually comes in what form?

A

dehydrated power

dissolves in water

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3
Q

what converts a liquid medium into a solid medium?

A

agar

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4
Q

how can media be sterilized?

A

autoclaving

filtration

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5
Q

what is a chemically defined medium?

A

composition and amount of each ingredient is known

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6
Q

what is a chemically undefined medium/complex media?

A

composition and amount of each ingredient is not known

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7
Q

what is an enriched medium?

A

additional nutrients added to encourage growth of fastidious (difficult to grow) MO

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8
Q

what is a differential medium?

A

differentiates b/w MO based on their ability to conduct a metabolic process

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9
Q

what is a selective medium?

A

contains ingredients that select for a particular type or group of MO

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10
Q

what is a maintenance medium?

A

allows for the maintaining of MO over a long period of time

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11
Q

what is agar composed of?

A

polysaccharides isolated from red algae

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12
Q

when does agar melt?

A

100 degrees Celsius

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13
Q

when does agar solidify

A

45 degrees Celsius

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14
Q

can agar be melted and solidified repeatedly?

A

yes

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15
Q

what are some important characteristics of agar?

A

Most MO cant use it as a carbon and energy source
converts a liquid medium into a solid medium
necessary to kill endospores

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16
Q

what is an autoclave?

A

fancy, expensive pressure cooker

17
Q

what is an autoclave used for?

A

sterilization of liquid media
glassware
metal ware

18
Q

what can not be used with an autoclave?

A

heat sensitive materials

19
Q

what are the conditions for reaching sterilization with an autoclave?

A

121 degrees Celsius
15 PSI
15 minutes

20
Q

what is one form of media?

A

liquid/

NUTRIENT broth

21
Q

what is another form of media?

A

semisolid (low concentration of agar added)

like jello

22
Q

what is the third form of media?

A

solid

1.5% agar concentration added

23
Q

what concentration of agar is needed t convert a broth/ liquid medium into a solid medium

A

1.5% agar

24
Q

what is a slant? refer to pic in p.p

A

angled in tube

25
Q

what is a deep?

A

agar in test tube and not angled

26
Q

what is a petri dish?

A

agar in a dish with two halves

27
Q

How do you prepare a medium/agar?

A

weigh agar
mix Agar
dispense agar
autoclave

*for agar plates, pour sterilized medium in petri dish

28
Q

how are endospores killed?

A

only in an autoclave

Micro prac 1 (17 decks)

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