3-10 Flashcards

1
Q

what endospore staining technique are we doing in lab?

A

Schaeffer -Fulton technique

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2
Q

what are endospores?

A

dormant form of bacterium that enables it to survive in poor environmental conditions

for example: desiccation, acidic or basic pH, extremes of temperature, low nutrient concentration, radiation, presents of toxins

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3
Q

what is a Genera that produce endospores?

A

Bacillus
Heliobacterium
Sporosarcina
Sporomusa

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4
Q

what Botulism caused by?

A

Clostridium botulinum

Gram + anaerobic Bacillus (food borne)

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5
Q

how toxic is Botulism?

A

produces a toxin so powerful that one pint of the pure material could eliminate the entire population of the world

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6
Q

what are symptoms of botulism?

A

blurred vision
slurred seech
difficulty swallowing/chewing
labored breathing develop with in hours

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7
Q

how is Botulism treated?

A

antitoxins

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8
Q

how can one prevent Botulism?

A

boil canned food for at least 10 minutes

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9
Q

what is tetanus caused by?

A

Clostridium tatani

one of the most dangerous disease

second most powerful toxin

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10
Q

how does tetanus come about?

A

spores enter a wound and revert to vegetative bacilli that produce toxin

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11
Q

what do the toxins of Tetanus provoke?

A

sustained and uncontrolled contractions of the muscle

violent death

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12
Q

how is Tetanus treated?

A

muscle relaxants
Penicillin
antitoxins

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13
Q

how is Tetanus prevented?

A

DPT vaccine 2,4,6 months

booster recommended every 10 years

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14
Q

what is Anthrax caused by?

A

Bacillus anthracis

Gram + (soilborne)
germinate rapidly
lesions covered with black crusts

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15
Q

who does Anthrax affect?

A

cattle
sheep
goats
(ingest spores from soil)

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16
Q

when animal dies, how does one get rid of Bacillus Anthracis ?

A

cremate/burn?

17
Q

how is Anthrax treated?

A

penicillin

18
Q

what is causes Gas Gangrene?

A

Clostridium perfringens

gram +

19
Q

what happens with Gas Gangrene?

A

blood flow ceases to a part of the body

body part becomes dry and shrunken skin color is purple to black

20
Q

symptoms of Gas Gangrene?

A

intense pain

swelling

foul odor

21
Q

treatment for Gas Gangrene?

A

amputation

22
Q

what are endospores composed of?

A

Exosporium- thin protein layer

cortex- loosely linked peptidoglycan

core- cell wall, cell membrane, cytoplasm

23
Q

spore morphology

A

central: center of cell
terminal: on end of cell
lateral: side of cell

24
Q

what are the results of endospore staining?

A

red (-) Staphylococcus aureus

green (+) Bacillus Megaterium

25
Q

steps for endospore staining

A

primary stain: Malachite Green

decolorization: water

secondary stain” Safranin

26
Q

when is sporulation done?

A

in response to nutrient depletion