Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Micro prac 1 > 2-7 > Flashcards

2-7 Flashcards

1
Q

how does the composition of Fluid Thioglycollate broth affect MO(s) based on their oxygen requirements?

A

enables MO(s) to grow at different places in the tube

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what is aerotolerance?

A

indicates the ability or inability of MO to grow in the presence of oxygen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what is an obligate aerobe?

A

need oxygen

grow top of tube

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what is facultative anaerobes?

A

grows with or without oxygen, but better with
makes ATP
grow top to middle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what is an aerotolerant anaerobe?

A

grows with or without oxygen, does not care for oxygen
grow top to middle
grow top to middle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what is an obligate anaerobe?

A

die in presence of oxygen
grow in absence of oxygen
grow bottom of tube

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what are microaerophiles?

A

MO that needs reduced amount of oxygen between 7-10/8-10% in order to grow
grow little below top

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what are capnohiles?

A

MO that requires carbon dioxide to grow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what does Fluid Thioglycollate medium contain?

A 
yeast extract
glucose
casein
methylene blue indicator
small amount of agar
sodium Thioglycollate
L-cysteine
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

what does yeast extract provide in Fluid Thioglycollate medium?

A

vitamins and minerals for bacterial growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what des glucose and casein provide in the Fluid Thioglycollate medium?

A

carbon and energy source

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

what does agar provide in he Fluid Thioglycollate medium?

A

slows diffusion of O2 into medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what does sodium Thioglycollate and L-cysteine provide in the Fluid Thioglycollate medium?

A

reduces free oxygen to water, thereby making oxygen unavailable

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what color is the methylene blue indicator when oxidized (presence of oxygen)?
reduced (absence of oxygen)?

A

oxidized: blue
reduced: colorless

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what is the indicator of aerobic or anaerobic conditions in Fluid Thioglycollate medium?

A

Resazurin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

what color is resazurin when oxidized? reduced?

A

oxidized: pink

reduced? colorless

17
Q

why does one NOT shake or mix Fluid Thioglycollate tubes?

A

If introduced to oxygen, it will exhaust the Fluid Thioglycollate.

18
Q

what kind of medium is Fluid Thioglycollate?

A

basic

19
Q

what is Fluid Thioglycollate used for?

A

promote growth of a variety of fastidious and nonfastidious MO(s)

illustrates microbial growth representing all levels of of oxygen tolerance

Micro prac 1 (17 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions