4 - Membrane Transport Flashcards
- restricts passage of most polar molecules
- different concentration of solutes in the cytosol and extracellular fluid
- membrane transport proteins
- transporters and channels
Membrane transport
2 main classes of membrane proteins
- Transporter (carriers)
- Channels
3 Solute carrier (SLC) transporters
- Uniporter
- Symporter
- Antiporter
An ABC transporters
pump
Principles of membrane transport
transport by carriers can be either active or passive, solute flow through channel proteins is always passive.
Moevement of solute in uniporter
facilitated diffusion
Symporter, antiporter, and pump move solute using active or passive transport?
Active transport
uses primary active transport
pump
Use secondary active transport
Symporter and antiporter
lipid bilayers are impermeable to ___
ions
The passage of molecules across membrane depends on ___ and _____
size and hydrophobicity
Example of hydrophic molecules
- O2
- CO2
- N2
- Steroid hormones
Example of small uncharged polar molecules
- H2O
- Urea
3.Glycerol - NH3
Example of large uncharged polar molecules
- glucose
- Sucrose
Example of ions
- H+
- Na+
- HCO3-
- K+
- Ca2+
- Cl-
- Mg2+
transfer solutes across cell
membranes
membrane transport proteins
- transfer specific molecular
species or a class of molecules - highly specific
transport proteins
Example genetic disorder that is due to single-gene mutation in transport proteins
cystinuria
- single-gene mutation
- autosomal-recessive defect in the reabsorptive transport of cystine from the urine or intestine into the bloodstream.
- Csytine accumulates in the urine. This build up leads to the formation of cystine stones in the kidney
cystinuria
All membrane proteins that have been studied in detail are ____ ____ ___
multipass transmembrane
proteins
means that their polypeptide chain cross the lipid bilayer multiple times
multipass transmembrane protein
pathway formed by membrane proteins which allows specific hydrophobic solutes to cross
protein-lipid pathway
transporters are also known as
carriers or permeases
- bind
- conformational changes
transporters
form continuous pores
channels
“downhill” transfer
passive transport
Movement of uncharged molecule across membrane is driven by
concentration gradient
Movement of charged molecule aross membrane is driven by
membrane potential
- uphill
- against their electrochemical
gradients
active transport
In most biological cells, the inside of the cell has ___ ___ ___
Negative electrical potential
negative
insided
electrical potential
Tightly coupled with energy
transporter
Process by which transporter transfer a solute resembles an ___-___ reaction
enzyme-substrate react
transporters have specific ___ sites for its solute
binding
transporters undergo a ___ ___ ___ after binding to their substrate molecules.
reversible conformational change
In some transporter proteins, an intermediate state called the ___ state exists during the transport cycle
occluded
represents a conformation where the binding site for the substrate is inaccessible from either side of the membrane.
Occluded state
3 Confirmational changes in transporters
- Outward-open
- Occluded
- Inward-open
3 main ways for cells to perform active transport
- Coupled transport
- ATP -driven pumps
- Light-or-redox-driven pumps
use energy stored in concentration gradients
Coupled transport
used energy from hydrolysis of ATP
ATP-driven pumps
Use energy from light
light- or redox-driven pumps
2 main type of transporter
- Uniporter
- Coupled transporters
- passive transport
- move one type of solute across membrane
uniporters
- transfer of one solute depends on the
transport of a second - move 2 different solute at the same time
Coupled transporters
2 types of coupled transporters
- Symporters
- Antiporters
- transfer 2 different solute in the same direction
Symporters
transfer 2 different solute in the opposite direction
Antiporters
harvest energy stored in the electrochemical gradient
Coupled transport
Example of coupled transporters
- Na+ - co-transported ion
- Na+-K+ pump
- Na+-driven symporters
- Na+ and glucose, and neurotransmitters
Example of symporter
Na+ - co-transported ion
Example of primary active transporter
Na+-K+ pump
Transporters have 10 or more ___ ____ that span
the membrane
a helices
are located midway through
the membrane
solute- and ion-binding sites
Allows the transporter to alternately open and close on other side of membrane
pseudosymmetric or inverted repeats
structure
Inverted repeats are also known as
pseudosymmetric
2 channels that evolved from coupled transporter in which gating mechanism were lost, allowing the channels to open simultaneously on both sides of the membrane
aquaporin water channel and
the Sec61 channel (ER)
3 classes of ATP-driven pumps
- P-type pumps
- ABC (ATP-binding cassette) transporters
- V-type pumps
-phosphorylates
-phosphorylate themselves during the transport cycle
P-type pumps
-pump small molecules
ABC (ATP-binding cassette) transporters
- turbine-like
- made from multiple different subunits
- pumps H+ ions into organelles like lysosomes, vacuoles, and synaptic vesicle
V-type pumps
structurally related to V-type pumps but function in reverse manner
F- type (ATP synthase)
use the H+ gradient across the membrane to drive synthesis of ATP
F-type (ATP synthase)
Pumps Ca2+ into the sarcoplasmic reticulum in muscle cells
P-type ATPase
pumps Ca2+ out of the cell
Ca2+ ATPase
intracellular storage of Ca2+ in
muscle cells
sarcoplasmic reticulum
release of Ca2+ into the cytosol (Ca+-release channels) can trigger ____ _____
muscle contraction