3.7 Mitosis Flashcards

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1
Q

Describe the stages of the cell cycle in euk cells

A
  1. Interphase
  2. Mitosis
  3. Cytokineses
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2
Q

What is interphase

A

The DNA replicates leading to 2 chromatids joined at the centromere
Number of organelles and volume of cytoplasm increases and protein synthesis

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3
Q

What is mitosis

A

The nucleus divides to produce 2 nuclei with identical copies of DNA produced by the parent cell

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4
Q

What is cytokineses

A

Cytoplasm and cell membrane divides to form 2 new geneticallyi identical duaghter cells

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5
Q

Name the 4 stages of mitosis

A

Prophase
Metaphase
Anaphase
Telophase

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6
Q

What happens in stage 1 of mitosis (3)

A
  • Prophase: Chromosomes condense, becoming shorter and thicker
    -They also appear as 2 sister chromatids joined by a centromere
  • Nuclear envelope breaks down
  • Centrioles move to oppoiste poles forming spindle network
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7
Q

What happens in stage 2

A
  • Metaphase:Spindle fibres attatch to chromosomes by their centromeres
  • Chromosomes align along the equator
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8
Q

What happens in stage 3 of mitosis

A
  • Anaphase:Spindle fibres shorten and contract
  • Centromere divides
  • pulling chromatids to opposite poles of the cell
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9
Q

What happens in stage 4 of mitosis

A
  • Telophase: Chromosomes uncoil beocming longer and thinner
  • Nuclear envelopes reform making two nuceli
  • Spindle fibres and centrioles break down
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10
Q

Describe how prok cells replicate (3)

A

Through binary fission:
*Replication of circular DNA
* Replication of plasmids
* Divison of cyroplasms to produce two daughter cells
-Single copy of circular DNA
-Variable number of copies of plasmids

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11
Q

Why do some Euk cells not undergo the cell cycle

A

WIthin multicelluar organisms, not all cells retain the ability to divde_e.g. neurons)
Only cells that do retain this ability go through a cell cycle
Nuerons do not have centrioles

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12
Q

What is the importance of Mitosis in the life of an organism

A

Parent cell dvides to produce 2 genetically identical daughter cells for:
* Growth of multicellular organisms by increasing cell number
Replacing Cells to repair damaged tissues
Asexual reproduction

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13
Q

Describe how viruses replicate (4)

A

Being non living, viruses do not undergo cell division:
* Attatchment proteins attatch to complimentary receptors on host cell
* They inject viral nucleic acid (DNA/RNA) into host cell
* Infected host cell replicates virus particles such as nucelic acid viral proteins/ enzymes
* virus is then assmebled and then released

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14
Q

Explain how Prophase can be identified

A

Chromosomes are visible bevcause they are condensing
They are randomly arrnaged due to no spindle activity

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15
Q

Explain how metaphase canb be identifed

A

Chromosomes are lined up on the equator of the cell as they are attching to a spondle fibre

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16
Q

Explain how anaphase can be identified

A

Chromatids are at poles of spindle
Chromatids are a vshape because they are being pulled apart at their centromeres by spindle fibres

17
Q

Explain how telophase can be identified

A

Chromosomes are in two sets one at each pole

18
Q

What are the rules of scinefic drawing

A

Has to look similar to the spcimen/ image
No skteching/shading only clear anmd continuous lines
Include a magnifcation scale
Label with staright uuncrossed lines

19
Q

WHat is a mitotic index

A

Proportion of cells undergoing mitosis( with visible chromosomes
mitotic index=Number of cells undergoing mitosis

20
Q

Suggest how to calculate the time cells are in a certain phase in mitosis

A

Number of cells in that phase/ total number of cells observed
Multiply by length of cell cycle

21
Q

Explain how to determin a reliable Mitotic Index from observed squashes

A
  • Count cells in mitosis in FOV
  • Count only whole cells
  • Divide this by the total number of cells in FOV
  • Repeat with at least 5 FOVs selected at random
  • Calculate a mean
22
Q

Three stages of interphase in detail

A

G1 and G2- Number of organelles and volume of cytoplasm increases, protein synthesis
S phase-DNA replicates semi conservatively