3.3 Microscopic measurements and calculations

Question 1

What is an eyepiece graticule

Answer 1

A glass disc that is placed in the eyepiece of a microscope
A scale is also etched onto the glass disc

Eye piece graticules are alwats used in light micrscopes only

Question 2

Typically how long is the glass disc and how many divisions are there

Answer 2

10mm long and is divided into 100 divisions

Question 3

Why can the scalle on the eyepiece gratiucle not be used directly to measure the size of objects under a microscopic objective lens?

Answer 3

Each objective lens will magnify to a different degree

Question 4

How do you calibrate the eyepiece graticule

Answer 4

A stage micrometer is needed (a type of microscopic slide)

Question 5

How long is the stage micrometer

Answer 5

2mm long and its smallest subdivsions are 0.01mm (10um)