3.6 Structure Of Proteins Flashcards
How many amino acids are there in an organism
20
How are amino acids joined together?
By condensation reactions
A peptide bond is formed between two amino acids, producing a dipeptide
A polypeptide is firmed when many amino acids bond together
What is the primary structure of a protein?
The polypeptide produced during translation
What is the secondary structure of a protein?
A coiled or pleated sheet protein structure
Alpha helix - coiled
Beta helix - pleated
What is the tertiary structure of a protein?
A specific 3D shape as a result of hydrogen bonds, ionic bonds, disulfide bridges and hydrophobic interactions
What is the quartenary structure of a protein?
When two or more polypeptides associate
What test identifies proteins?
The biuret test
How is the biuret test carried out?
- Add sodium hydroxide (NaOH) to the sample (equal volumes)
- Add drops if copper sulphate solution (which is blue) and mix
- A purple colour shows the presence of protein
What is the difference between DNA and ATP?
-The base in ATP is always adenine whereas in DNA the base is either adenine, thymine, cytosine and guanine
-There are 3 phosphate groups in ATP and only one in DNA
-The sugar in ATP is ribose but in DNA it is deoxyribose
(Similarities both contain a nitrogenous base and a sugar. They also have phosphate groups involved