3- Public Health Microbiology

Question 1

Provide the equation used to work out the number of samples required in an outbreak investigation?

Answer 1

100/target prevalence of that pathogen in flock x 3

Question 2

What temperature should samples be kept at in a disease outbreak case?

Answer 2

4 degrees = use a portable fridge

Question 3

When is direct plating used as a lab test?

Answer 3

When you suspect a robust pathogen in large quantities

Question 4

When dealing with a low or fragile organism which of the following lab tests is ideal?

Direct plating, enrichment, Tissue culture or molecular?

Answer 4

Enrichment culture

Question 5

What should you see in a positive salmonella agglutination test?

Answer 5

Granularity after 1-2 mins

Question 6

What does pulsed field gel electrophoresis do?

Answer 6

Produces a DNA fingerprint for a bacterial isolate

Separates DNA fragments according to size

Samples whole genome

Shows patterns that is specific for particular strain