18. Rhabdoviridae

Question 2

Pathogenic rhabdoviruses of warm-blooded animals (3 genera)

Answer 2

Ephemerovirus

Lyssavirus,

Vesiculovirus

Question 3

What viruses have the following properties:

Virions are bullet shaped, 45-100 nm in diameter and 100-430nm long and consist of an envelope with large spikes within which is a helically coiled cylindrical nucleocapsid

The genome is a single molecule of linear, negative-sense, single-stranded RNA, 11-15 kb in size

Cytoplasmic replication

Viral RNA-dependent RNA polymerase (transcriptase) transcribes five subgenomic mRNAs, which are translated into five proteins:

1. L, the RNA-dependent RNA polymerase (transcriptase);
2. G, the glycoprotein that forms the envelope spikes;
3. N, the nucleoprotein, the protein that associates with RNA to form the viral nucleocapsid;
4. P, a phosphoprotein that mediates binding of L protein to the nucleocapsid;
5. M, which associates with the viral nucleocapsid and lipid envelope

Maturation is by budding through the plasma membrane

Answer 3

Rhabdoviruses

Question 5

MEMBERS OF THE GENUS EPHEMEROVIRUS (Fam. RHABDOVIRIDAE) (1)

Answer 5

BOVINE EPHEMERAL FEVER VIRUS (also called 3-day stiff-sickness)

Question 6

MEMBERS OF THE GENUS LYSSAVIRUS (Fam. RHABDOVIRIDAE) (1 species)

Answer 6

RABIES VIRUS

RABIES-LIKE VIRUSES (European bat lyssaviruses 1 and 2, and Australian bat lyssavirus)

Question 7

MEMBERS OF THE GENUS VESICULOVIRUS (Fam. RHABDOVIRIDAE) (1 species)

Answer 7

VESICULAR STOMATITIS VIRUS