(18) Innate Immunity Flashcards
1
Q
look at this image a bit for review of what belongs where
A
2
Q
- What is the most abundant phagocyte in the blood?
A
- neutrophil
3
Q
(Macrophages)
- role in what?
- secrete what?
A
- phagocytosis and killing of bacteria
- pro-inflammatory cytokines
4
Q
(eosinophils)
- good for fighting what?
- Why?
- What happens when they crosslink?
A
- parasitic infections
- have Fc receptors for IgE
- dump out a lot of toxic products to kill parasite
5
Q
(Mast Cell)
- when surface IgE is cross-linked by binding to Ag - what will occur?
A
- mast cells will degranulate, dumping out toxic and proinflammatory products
6
Q
- What is the process by which particles in extracellular fluid are taken up by phagocytes?
- receptor-mediated or non-specific uptake?
- What are the 3 major phagocytes?
A
- phagocytosis
- either
- macrophages, neutrophils, dendritic cells
7
Q
- Once a bacterium is engulfed in phagocytosis, for anything good to happen, one must happen following this?
- What is responsible for this acidification?
- What is the intracellular pocket created after phagocytosis called?
- What is the purpose of the granules?
A
- acidification
- NADPH oxidase
- phagosome (like the endosome)
- They contain the enzymes that fuse with the phagosome and degrade it
8
Q
(Respiratory Burst)
- What is this?
- What are the five products?
- What enzyme makes hydrogen peroxide?
- What enzyme makes hypochlorite?
- How do ROP damage stuff?
- Doesn’t start until what?
- Which thing’s macrophages make very little ROP?
A
- When the cell uses oxygen to make reactive oxygen species
- superoxide anion, hydrogen peroxide, singlet oxygen, hydroxyl radical, and hypochlorite
- superoxide dismutase
- myeloperoxidase
- bind to membranes
- until NADPH oxidase is activated by acidification of the vesicle
- avians
9
Q
(Reactive nitric oxide)
- occurence in different species?
- What is the equation to make it (including enyzme)
- Does it react in the same way the ROP do?
A
- humans and pigs a little, cattle some, rodents a lot!
- O2 + L-arginine = NO + citrulline
enzyme is NO synthetase
- yes!
10
Q
(Natural Killer Cells)
- What is ADCC?
- How does this work?
- Attacks what cells primarily?
A
- Antibody dependent cellular cytotoxicity
- receptors on NK cells that crosslink when they encounter antibodies on cell that triggers NK cell to dump out its contents and kill the target cell
- tumor cells and virally infected cells (also some bacteria)
11
Q
- Why are NK cells needed when CD8 does the same thing?
- So what serves as a signal for NK cell attack?
- What will happen to animals deficient in NK cells?
- What do NK cells secrete?
- What happens when NK cells are stimulated by IFN-a and IFN-b (released by virally infected cells)?
- What happens when they are stimulated by IL-12?
A
- some cancers escape immunodetection by shutting down MHC I expression - NK cells can deal with these kinds of cells
- lack of expression of MHC class I
- get sick more cause recurrent viruses that inhibit MHC class I expression won’t be dealt with
- Pro-inflammatory cytokines (IFN-y and TNF-a)
- cell killing
- favors cytokine secretion (IFN-y and TNF-a)
12
Q
- When NK cell encounters MHC class 1 what happens?
- What happens if there is no MHC class 1?
- Do NK cells have a T cell receptor?
A
- a negative signal is sent and NK cell doesn’t do anything
- positive signal; it’s on
- no
13
Q
- What are the things that send positive signal to NK cell?
- When are they expressed?
- So what if there are both MHC class I and the aforementioned signals?
A
- MIC-A and MIC-B (carbohydrate ligands)
- under times of stress
- depends on the balance of signals it is receiving
14
Q
(NK cells and ADCC)
- What kinds of cells do NK cells kill?
- What happens in ADCC?
- Why is this important?
A
- self cells
- Fc receptors on the surface of the NK cell get crosslinked when they bind antibody on cell - release same things as Tc then (perforin, granzymes, etc.)
- Tc takes awhile - NK cells can act faster
15
Q
(Innate Lymphocytic Lineage Cells)
- several lymphocyte lineages behave as innate like cells
- have antigen-specific receptors (don’t undergo clonal expansion)
1. most are derived when?
2. What one are we going to worry about?
3. are they part of immne response?
4. Do they release cytokines?
5. What do they recognize?
6. Does it have a T-cell receptor? how is it different?
A
- early in life
- gamma-delta T cells
- yes
- yes (IL-23)
- MHC class IB (an unusual one) - aka MIC-A and MIC-B
- yes; doesn’t see antigen directly (doesn’t require antigen presentation)