(11) T-cell II Flashcards
(T cell differentiation in the thymus)
- CD4 and CD8 are proteins on T cells that are associated with what?
- What is a protein associated with the TCR and provides the singals to the T cell when TCR binds to MHC-ag complex?
- How many T cells are lost in thymus?
- The TCR
- CD3
- 95%
- Which chain of TCR rearranges first?
- mature T cells leave the thymus via what?
- does t-cell production occur constitutively?
- the beta chain, then alpha
- the blood
- yes

(T cell trafficking)
- Once T cells enter the lymph tissue - what can they encounter?
- What happens if they don’t?
- What happens to T cells that do encounter their Ag?
- Once activated, where do they go?
- does t-cell receptor rearrange completely independent of antigens?
- Ag presenting cells
- reenter circulation via lymphatics
- activated to proliferate and differentiate into armed effector cells
- leave via lymphatics and circulate to other lymphoid organs, and tissues depending on what cytokines and chemokines they encounter
- yes (part of development, not activation)
just read this and get general idea (on ipad)

(T cell maturation in the periphery)
- Adaptive immune response starts by co-mingling of different cell types where and where?
- What brings antigen to the lymph nodes?
- What can it do there?
- What are also present than interact with expanding T cells?
- To become activated, T cell must receive multiple signal from what?
- What are these signals?
- draining lymph nodes and other secondary lymphoid organs
- APC (dendritic cells)
- can attract T cells via chemokine production (T cells will sample the presented antigens on the surface of the APC)
- B cells
- The APC
- TCR must be engaged, co-stimulatory receptor-ligand interactions must occur, and cytokines must stimulate T cell
T helper cells exist in functionally distinct suspopulations:
- What do Th1 do?
- Th2?
- Th17?
- activate phagocytes and amplify innate immune responses
- activate B cells and amplify humoral immune responses
- activate neutrophils
just read this…


(Th Cell Subpopulations)
- Are CD4 cells leaving the thymus naive?
- During activation in the lymph node they can become what of four types?
- They exit the thymus as what? proliferate in response to what? then differentiate into what?
- Pathway is controlled by what?
IL-12 and IFN-gamma causes what?
TGF-beta and IL-23 and IL-6?
IL-10 and IL-4?
- Each Th cell subpopulation has different role in what?
- no
- Th1, Th2, Th17 (or Treg cells)
- uncommitted Th0 cells; ag presentation; one of the subpopulations
- cytokines
TH1
TH17
TH2
- adaptive immunity
(Th Cell Differentiation)
- How can the pathogen influence which T cell population differentiates?
- Viruses and some bacteria induce what secretion by dendritic cells? produce what?
- Worms induce what? Cause what?
- by influencing the cytokines produced (during the inital interaction with non-specific immune cells)
- IL-12 and IFN-gamma ; Th1
- IL-4; TH2
(Th1 Cells)
- What is the principal function of Th1 cells?
- Secrete cytokines that augment what?
- Secrete factors that inhibit what?
- macrophage activation
- augment non-specific immune responses
- Th2 differentiation
(just look at list)
GC-CSF promotes granulocyte and macrophage production in bone

remember these functions and factors that promote them - don’t kill yourself over it though - main thing to remember is that Th1 make “macrophages” happy


- What is the primary activity of Th2 cells?
- Efficient activators of macrophages?
- Secrete factors that regulate isotype switching? if so which ones?
- Secrete factors that inhibit what? what specifi factors accomplish this?
- to activate and differentiate B cells
- no
- yes, IL-4 and IL-5
- inhibit Th1 differentiation; IL-4 and IL-5
(Th2 cells influence IgE class switch)
- IgE class switching in B cells is initiated by what cells? these develop in the presence of what?
- The presence of what regulates isotype expression?
- What is the first signal sent to Th0 cell through? second? third?
- Do we always understand what drives production of certain cytokines? In some cases, the same pathogen can induce different responses (cytokines) depending on form or anatomic location
- Th2, IL-4
- cytokines
- TCR MHC molecule, co-stimulatory molecules, cytokines
- no
(Th17 cells)
- What cytokine is produced by Th17 cells? is it pro or anti-inflammatory? Play an important role in what?
- IL-17; pro-inflammatory; against extracellular microbes and promoting neutrophil recruitment (to target extracellular bacteria)
(Cytotoxic T Cells (Tc or CTL))
- Do Tc interact with target cells bearing a specific antigen? Do they use the same receptor system as CD4? What is the only difference?
- These are particularly important fighting what?
- Will they kill surrounding cells of host?
- Do they have rearranged TCR like Th cells do?
- yes; yes; CD4 molecule replaced by CD8 homodimer
- viral infections and intracellular bacteria
- no
- yes
(Tc Cells)
- What are the three cytotoxins the Tc granules release to kill cells?
- perforin, granzymes, and lymphotoxin-alpha (LT-a)
(Tc)
- What does perforin do?
- What do granzymes do?
- What do lympotoxin-alpha do?
- polymerizes on cell membrane to form pore
- serine proteases which cleave proteins on target cell membrane
- induces cell death via apoptosis (cell death by cleaving host DNA)
(Tc Cells)
- Are neighboring cells damaged when Tc cells kill stuff? What account for this specificity? Does this process damage Tc cells themselves?
- How do Th cells augment Tc cells?
- What do CD8+ cells secrete what that cna block viral replication?
- do apoptotic cells trigger further inflammation?
- Granzymes activate what in target cells to start apoptosis?
- Do cytotoxic T-cells make cytokines that make target cells more susceptible to apoptois?
- no; polar release of the cytolytic granules (release contents only in the space between the Tc cell and target); no (they can go to another cell)
- secretion of IL-2 and by increasing MHC class 1 gene expression
- IFN-a
- no
- capases
- yes
(Memory T cells)
- After an immune response, the number of T cells responsive to the antigen increase how many times? These live a long time and are called what? Does this occur for both CD4 and CD8? How are they distinguished?
- Do memory T cells hang out in one place like most memory B cells do (in the bone marrow)?
- 100x; Memory T cells; yes; by surface proteins
- nope - some produce cytokines and enter inflamed tissue - some express CCR7 and hang out in lymph nodes (don’t really need to remember these protein names)
- Are memory T cells like naive T cells? what’s the difference?
- What are some benefits of some of the proteins that memory T cells express?
- no, alot easier to activate memory T cells
- gives you stronger receptor signalling, prolonged survival, or trafficking to certain tissues
(understand this)
- Are Th cells required to make and activate memory T cells?
- the thing on the next page shows this in experimental form

- yes
