14 Flashcards
Active receptor starts a chain of
events where messages are passed
on through the cell via a process
called
signal transduction.
How can a message be passed on? In signal transduction
The message can be passed on
using proteins, chemical signals
called second messengers , or
through sequential phosphorylation.
What uses second messengers?
Many different receptors, especially G protein-coupled
receptors use second messengers.
How do second messengers work?
Second messengers can transmit signals from a receptor
to other relay molecules because they are not attached to
the membrane and are free to move in the cell.
What is phosphorylation
Widespread mechanism for regulating protein activity where
protein kinases transfer phosphates from ATP to protein
(phosphorylation).
What rapidly removes protein phosphates from proteins
Protein phosphatases rapidly remove the phosphates from
proteins (dephosphorylation) to carefully control signal transduction.
What does signal transduction pathways use to relate a phosphorylation cascade
Signal transduction pathways often use many different
protein kinases, creating a phosphorylation cascade.
What are GPCRS? What do they use to start signal transduction?
G protein-coupled receptors (GPCRs) use G proteins to
start signal transduction, hence their name.
What activates G protein
The GPCR activates the G protein, which communicates
with other proteins in the cell.
The different types of G proteins and the different effects
Gα s = stimulatory G protein, which activates an enzyme called adenylate cyclase Gαi = inhibitory G protein, decreases the activity of adenylate cyclase
Signal transduction usually contains…
- multi-step pathways that provide opportunities for co-ordination and regulation of the cellular response
What does phosphorylation and dephospylation do?
Phosphorylation and dephosphorylation events turn protein activity on and off or up to down as required
How is signal transduction tightly regulated to control cellular activity?
- Ligand dissociation (makes receptor inactive)
- Internalisation - receptor is removed from the cell surface through endocytosis so it can no longer respond to ligand
- Phosphatases - (removal of group to turn off activity of a kinase)
What does G protein stand for?
Guanine nucleotide binding protein
Structure of G-protein
Heterotrimerie - 3 different subunits
Which subunit is most important for signal transduction
- alpha subunit
What is the G-protien cycle?
- the way the G protein is activated and inactivated
- exchange of GTP and GDP on guanine nucleotides
Different types of _____ ______ produce different ________
Different types of alpha subunits produce different signalling
Diagram of singnal transduction for a ‘Gs-coupled’ GPCR - hand drawn
Diagram of singnal transduction for a ‘Gs-coupled’ GPCR
Glucagon receptor signal transduction
GLP-1 receptor signal transduction
Signal transduction for RTKs - diagram of structure
RTK example - insulin receptor signal transduction - muscle and adipose
- glut 4 (glucose transporter) gets translocated to cell surface
- receptor activation causes phosphorylation of an “adaptor” protein, and further signal transduction events, leading to GLUT-4 transocation
- muscle and adipose cells
- insulin is an example of a peptide ligand
Insulin receptor transduction in liver cells
- in liver cells, receptor activation causes phosphorylation of an “adaptor” protein, and further signal transduction events, this time leading to glycogen synthesis
- different things can happen in different cell types
Digaram the illustrates how cells can produce different responses (4 diff cell types) - YAPP
- Receptor is activated by ligand producing a particular chain of events leading to a cellular response
2+3. Same receptor is expressed and responding to same ligand but get a different response due to pathway branching where receptor activates first same pathway protein but then goes on to two different down stream proteins in this particular cell type resulting in a unique response - Two different receptors coming into play which co-ordinate each others activities - second receptor activates its own unique signal transduction pathway which has cross talk with signal transduction pathway of the other receptor leading to a unique response
- Second receptor activates its own chain of events leading to a unique response
