1.11 Oxidative deamination of amino acids

Question 1

Give the general reaction of oxidative deamination of Aas

Answer 1

Aa → α-keto acid + NH₃

Aerobic conditions

Question 2

Location of oxidative deamination

Answer 2

All tissues

Mitochondrial matrix

Question 3

Give the reaction of oxidative deamination of glutamate

Answer 3

Glutamate dehydrogenase

Question 4

How is oxidative deamination regulated?

Answer 4

Allosterically:

Glutamate dehydrogenase:

• ADP (+)
• ATP (-)

Question 5

L-Amino acids

Answer 5

• Located in the Kidney + Liver
• Use FMN
• Gly, Asp

Question 6

D-Amino acid oxidases

Answer 6

• Defense system
• Aerobe dehydrogenases → use FAD
• All D-amino acids have their own oxidase
• Irreversible

Question 7

Draw the diagram showing the central role of glutamate

Answer 7

Question 8

Draw the diagram giving the fate of nitrogen-free carbon chains of amino acids

Answer 8

Question 9

Transamination of amino acids: General reaction

Answer 9

Amino acid (-NH₂) ⇔ α-ketoacid (=O)

Transaminase

Question 10

Which amino acids does transaminase not affect?

Answer 10

• Thr
• Lys
• Pro

Question 11

Draw the schiff-base mechanism

Answer 11

Question 12

Draw transamination using AST

Answer 12

AST = Aspartate aminotransferase

Question 13

Where is AST active?

Answer 13

• Liver
• Heart
• Skeletal muscle

Question 14

Draw transamination using ALT

Answer 14

ALT = Alanine aminotransferase

Question 15

Where is ALT active?

Answer 15

Liver