11.1 - 11.3 Quiz Flashcards
Virus
Nucleus acid (DNA or RNA) wrapped in protein, not made of cells, reproduce using a cell
Transforming substance
Found in bacteria by Griffith
Bacteriophage
Virus that infects bacteria
DNA replication
Process of copying DNA molecule
Template mechanism
DNA molecule serves as template to make an identical copy
Origin of replication
First step in double helix replication, needed because we need to be specific about what strands are being replicated.
Replication Bubbles
Push outward in both directions, opening parent DNA strands
Parent DNA stands
What is expanded by the replication bubbles
Pyrimidine
Single ring base (T and C)
Purine
Double ring base (A and G)
Daughter DNA Strands
Formed with complementary bases added opposite parent strands
DNA polymerase
Enzyme which forms covalent bonds between nucleotides in daughter strand
Double Helix
Twisting shape of 2 connected strands of nucleotides
Complementary base pairs
Made of nitrogenous bases, based on size of bases, A-T, G-C, sequence determines complementary sequence of other strand
Name the four nitrogen bases
Thymine, Cytosine, Adenine, Guanine
Griffith’s experiment
Found a transforming substance which causes a heritable change in other bacteria, observed that bacteria transformed when mixed with deadly bacteria. Ended up killing mice.
Avery’s experiment
Preformed experiment with bacteria to investigate which molecule (DNA or protein) contains heritable genetic information. Found that DNA destroyed traits could not pass from one bacteria to another. DNA is the transforming factor
Hershey and Chase’s experiment
Used viruses to test protein vs. DNA as heritable factor. Group 1: mark protein with radioactive substance Group 2: mark DNA. Infect bacterial cells > DNA showed up inside the cells protein only show up outside the cells. They found that DNA is hereditary material.
Which bases are complementary?
A bonds with T, G bonds with C
How many bonds are shared between each base pair?
A and T: 2
G and C: 3
How does DNA replicate? (4 steps)
- Replication begins at origin of replication
- 2 strands separate
- Replication bubbles push outward in both directions, opening parent DNA strands
- Daughter strands formed with complementary bases added opposite parent strands. (DNA Polymerase added)