11: Antigen Presentation

Question 1

3 APC cells

Answer 1

dendritic cells, macrophages, B cells

Question 2

Which MHC class uses proteosomes?

Answer 2

I

Question 3

Which MHC class uses phagolysosomes?

Answer 3

II

Question 4

What transports peptide fragments into the ER?

Answer 4

TAP transporter

Question 5

MHC class I antigen presentation steps

Answer 5

1. proteins are degraded in the proteosome
2. peptides are transported from cytosol into ER
3. peptides associate with MHC class I
4. MHC class I molecule is exported to Golgi
5. MHC class I molecule is moved to cell surface
6. ligand interaction with CD8+ T cells

Question 6

Which MHC class assembles with beta 2-microglobulin?

Answer 6

I

Question 7

MHC class II antigen presentation steps

Answer 7

1. binding of antigen
2. endosome formation
3. endosome pH decreases to activate proteases
4. endosome-lysosome fusion contains more proteolytic enzymes and MHC class II molecules
5. association of processed Ag with MHC class II
6. fusion of phagolysosome with plasma membrane
7. ligand interaction with C4D+ T cells

Question 8

Which MHC class is associated with APCs?

Answer 8

II

Question 9

B cell antigen presentation steps

Answer 9

1. Ig on surface of B cell binds to epitopes on native antigen
2. Ig “caps” and the antigen is internalized
3. the antigen is processed similarly as in macrophages
4. peptide-MHC class II complex is exported to the cell surface
5. ligand interaction with CD4+ T cells

Question 10

T/F: Plasma cells process and present Ag via MHC class II

Answer 10

F

Question 11

T/F: B cells process and present Ag via MHC class II

Answer 11

T

Question 12

What are the most potent stimulators of naive T cells?

Answer 12

dendritic cells

Question 13

Exoxomes

Answer 13

shed by dendritic cells and contain the antigen