(00) Brucellosis 1

Question 1

(Brucella)

1. have predilection for what in sexually mature animals?
2. gram?
3. oxygen?
4. grows where in body?
5. all species sim or different?
6. speciation performed by growing on agar with what?

Answer 1

1. female and male repro organs
2. negative (small rods)
3. aerobic or microaerophilic

4. facultative intracellular - macrophages and endothelial cells

5. very similar

6. basic fuchsin and thionin

Question 2

(Brucella)

(infection and path)

1. associated with what type of infections?
2. what do ruminants have a lot of in repro that supports gworth of this?
3. are they potential pathogens for humans?
4. WW

Answer 2

1. repro tract and abortions

2. erythretal (we humans don’t have as much)

3. yes (all species)

Question 3

(Brucella Abortus)

1. in what animals?
2. aka what in cattle?
3. what serve as reservoirs?

4. easily killed by what?

5. shed in large quantities of milk, urine, placenta

Answer 3

1. cattle, bison (wild-bison, elk, wolf, coytoe)
2. Bang’s disease
3. wild ruminants

4. pasteurization and disinfectancts

Question 4

(Brucella Abortus)

1. stimulated for growth by what?
2. requires how much CO2?
3. how common is US cattle herds?

what pops have high rates of infection?

4. transmission is primarily through what?
5. once animals ingests what is route?
6. most common route of infection is via what?
7. Animals that have aborted will do what?
8. in males causes what?

9 .what specifilaly causes abortion?

Answer 4

1. erythritol (human placenta does not have this)
2. 5-10%
3. almost completely eradicated

bison/elk

4. contaminated or untreated milk (also direct, carcass, placenta, feed)
5. lymph nodes –> bloodstream –> uterus, placenta, udder
6. GI tract
7. shed large amounts and contaminate envrinoment
8. inflammation of testicles (orchitis) and epididymis –> reduced semen quality (can be present in semen)

(localizes in mammary glands in females)

9. placentitis

Question 5

(Brucella Abortus)

(horses)

1. cause what?
2. cause abortion?
3. horses get infection from what?
4. can man get from horses?
5. are there cases of horse –> horse?

Answer 5

1. poll evil and fistulous withers

2. rarely

3. cattle or swine
4. yes - from open lesions
5. unknown

Question 6

(Brucella Aborthus)

(Humans)

1. can enter via what?
2. causes what?
3. treat with what?

Answer 6

1. cuts (common in vets/farmers)
2. undulant fever (range from mild to severe)
3. doxycycline and streptomycin

Question 7

(Brucella Abortus)

1. Diagnosis?
2. what are not vaccinated?
3. Animals are not typically treated but removed… why?
4. what vaccine is used?

at what age?

5. what becomes lifelong carrier?

so do what with it?

Answer 7

1. clinical signs, direct smears

keep plates for several weeks to ensure no grwoth

serum agglutination tests

milk ring test (mix milk with antigen)

card test - mix blood with antigen

2. bulls and pregnant cows (only liscenced vets can give vaccine)
3. treatment is not practical
4. RB51 - provides protection without showing up in serological tests (earlier either caused abortion or showed up in serological tests)

4-10 months

5. bull

remove it