WHOLE LESSON: IDENTIFYING BACTERIA Flashcards
- gram + cocci/ resimble grapes 1 dm
- facultative anaerobes, oxidase - , catalase +
staphylococcal species
staphy grow on?
non-enriched media
staphylococcal is ?
non-motile & non spore forming
staphy is a commensal on:
skin & mucous membranes
staphy is stable in?
environment
what is the colonial characteristcs of staphy:
-white opaque 4mm dm
-bovine & human strain: golden yellow
-cogaulase negative: pigmented
-gram+ , for chains & 1.0 dm
-catalase -, facultative anaerobes, non-motile
Staphylococcus species
colonies of staphylococcus are:
- small
- haemolytic
- translucent
pathogenic strains of staphy is:
- thick capsules
- produce mucoid colonies
where is the usual habitat of staphy?
- upper respiratory
- lower urogenital tract
staphy is suspectible to?
dessication
- gram+ bacteria w/ many branching filaments
-slow growth on laboratory media
-an opportunistic pathogen producing diverse inflammatory response
Genus actinobacteria
genus actinobacteria can be:
- anaerobic
- facultative anaerobic
genus actinobacteria is:
nonspore-forming & non-motile
actinobacteria is modifed by?
Ziehl-neelsen-negative
actino b. can colonize?
mucous membrane of animals
actino require?
enriched growth media for growth
this structure are part of the host response to this chronic infection
Club-shaped structure
-gram+
- aerobic & non-motile
-spores is from aerial filaments
-spoil saprophytes
- long, slender, branching filament w/ tendency to fragment into rods & cocci in smears
Nocardia species
growth of nocardia species is on:
Sabouraud dextrose agar
nocradia species modified _______ due to mycolic acid in cell wall
Ziehl-Neelsen-Positive
- gram+, filamentous & branching
-motile coccal zoospores about 1.5 dm
-aerobic & capnophilic
dermatophilus congolensis
d. congolensis has no growth on?
Sabouraud dextrose agar
d. congolensis can be found in:
foci on skin of carrier animals
______ is associated w/ equine nocardiform placentits, leading to loss of the foal in approx. 50% of cases
Crossiella equi.
- Gram-positive, pleomorphic bacteria
- Fastidious, requiring enriched media
- Occur singly, in palisades of parallel cells and in
angular clusters resembling Chinese characters
in stained smears. - commensals on mucous membranes
- Cause pyogenic infection
- non-motile facultative anaerobes
- catalase+, oxidase negative -
usual habitat: mucous
: Corynebacterium species
- named Corynebacterium equi
- Gram+ rods or cocci,
- aerobic, soil saprophyte, worldwide distrib.
- Non-motile, catalase +, oxidase - & weakly acid-fast
- oxidation-fermentation test & in sugar
fermentation tests negative - CAMP-test positive
- Respiratory pathogen of foal (under 6 m
Rhodococcus equi
Rhodococcus equi together with Nocardia sp and Mycobacterium sp they form?
Actinomyce
- Most are small, Gram-positive, coccobacillary
rods, up to 2 μm in length - Catalase +, oxidase-, motile, facultative anaerobic
- Environmental saprophytes
- Grow on non-enriched media
- Tolerate wide temperature & and pH ranges
- Tumbling motility at 25°C
- Aesculin hydrolysed
Listeria species
is a microbiological test used to identify the presence of hemolytic activity, particularly in the identification of certain bacteria. The test is named after the researchers who developed it.
CAMP test, or Christie-Atkins-Munch-Peterson test,
- gram+, small rods (smooth forms) or filaments (rough form)
-growth on non-enriched media
-complete haemolysis in 48 hours - catalase- & coagulase +
-Non-motile, oxidase -, facultative anaerobe - H2S formed along stab line in triple sugar iron agar
- found in porcine tonsils
Erysipelothrix rhusiopathiae
-Large, Gram-positive rods (but few are Gram-negative)
- Endospores produced
- Aerobes or facultative anaerobes
- Growth on non-enriched media
- Most species motile, catalase-positive and
oxidase-negative
- Majority are non-pathogenic environmental
organisms
- Bacillus anthracis (non-motile) causes anthrax
- Bacillus licheniformis is implicated in sporadic abortions in cattle and sheep
- Usual habitat: environment
Bacillus species
Large, Gram-positive rods
Endospores produced Anaerobic, catalase-negative and oxidase- negative
Motile (except C. perfringens)
Enriched media required for growth
Colonies of C. perfringens
surrounded by zones of double
haemolysis
Present in soil, in alimentary tracts
clostridium species
