WHOLE LESSON: IDENTIFYING BACTERIA

Question 1

• gram + cocci/ resimble grapes 1 dm
• facultative anaerobes, oxidase - , catalase +

Answer 1

staphylococcal species

Question 2

staphy grow on?

Answer 2

non-enriched media

Question 3

staphylococcal is ?

Answer 3

non-motile & non spore forming

Question 4

staphy is a commensal on:

Answer 4

skin & mucous membranes

Question 5

staphy is stable in?

Answer 5

environment

Question 6

what is the colonial characteristcs of staphy:

Answer 6

-white opaque 4mm dm
-bovine & human strain: golden yellow
-cogaulase negative: pigmented

Question 7

-gram+ , for chains & 1.0 dm
-catalase -, facultative anaerobes, non-motile

Answer 7

Staphylococcus species

Question 8

colonies of staphylococcus are:

Answer 8

1. small
2. haemolytic
3. translucent

Question 9

pathogenic strains of staphy is:

Answer 9

1. thick capsules
2. produce mucoid colonies

Question 10

where is the usual habitat of staphy?

Answer 10

1. upper respiratory
2. lower urogenital tract

Question 11

staphy is suspectible to?

Answer 11

dessication

Question 12

• gram+ bacteria w/ many branching filaments
  -slow growth on laboratory media
  -an opportunistic pathogen producing diverse inflammatory response

Answer 12

Genus actinobacteria

Question 13

genus actinobacteria can be:

Answer 13

1. anaerobic
2. facultative anaerobic

Question 14

genus actinobacteria is:

Answer 14

nonspore-forming & non-motile

Question 15

actinobacteria is modifed by?

Answer 15

Ziehl-neelsen-negative

Question 16

actino b. can colonize?

Answer 16

mucous membrane of animals

Question 17

actino require?

Answer 17

enriched growth media for growth

Question 18

this structure are part of the host response to this chronic infection

Answer 18

Club-shaped structure

Question 19

-gram+
- aerobic & non-motile
-spores is from aerial filaments
-spoil saprophytes
- long, slender, branching filament w/ tendency to fragment into rods & cocci in smears

Answer 19

Nocardia species

Question 20

growth of nocardia species is on:

Answer 20

Sabouraud dextrose agar

Question 21

nocradia species modified _______ due to mycolic acid in cell wall

Answer 21

Ziehl-Neelsen-Positive

Question 22

• gram+, filamentous & branching
  -motile coccal zoospores about 1.5 dm
  -aerobic & capnophilic

Answer 22

dermatophilus congolensis

Question 23

d. congolensis has no growth on?

Answer 23

Sabouraud dextrose agar

Question 24

d. congolensis can be found in:

Answer 24

foci on skin of carrier animals

Question 25

______ is associated w/ equine nocardiform placentits, leading to loss of the foal in approx. 50% of cases

Answer 25

Crossiella equi.

Question 26

• Gram-positive, pleomorphic bacteria
• Fastidious, requiring enriched media
• Occur singly, in palisades of parallel cells and in
  angular clusters resembling Chinese characters
  in stained smears.
• commensals on mucous membranes
• Cause pyogenic infection
• non-motile facultative anaerobes
• catalase+, oxidase negative -
   usual habitat: mucous

Answer 26

: Corynebacterium species

Question 27

• named Corynebacterium equi
• Gram+ rods or cocci,
• aerobic, soil saprophyte, worldwide distrib.
• Non-motile, catalase +, oxidase - & weakly acid-fast
• oxidation-fermentation test & in sugar
  fermentation tests negative
• CAMP-test positive
• Respiratory pathogen of foal (under 6 m

Answer 27

Rhodococcus equi

Question 28

Rhodococcus equi together with Nocardia sp and Mycobacterium sp they form?

Answer 28

Actinomyce

Question 29

• Most are small, Gram-positive, coccobacillary
  rods, up to 2 μm in length
• Catalase +, oxidase-, motile, facultative anaerobic
• Environmental saprophytes
• Grow on non-enriched media
• Tolerate wide temperature & and pH ranges
• Tumbling motility at 25°C
• Aesculin hydrolysed

Answer 29

Listeria species

Question 30

is a microbiological test used to identify the presence of hemolytic activity, particularly in the identification of certain bacteria. The test is named after the researchers who developed it.

Answer 30

CAMP test, or Christie-Atkins-Munch-Peterson test,

Question 31

• gram+, small rods (smooth forms) or filaments (rough form)
  -growth on non-enriched media
  -complete haemolysis in 48 hours
• catalase- & coagulase +
  -Non-motile, oxidase -, facultative anaerobe
• H2S formed along stab line in triple sugar iron agar
• found in porcine tonsils

Answer 31

Erysipelothrix rhusiopathiae

Question 32

-Large, Gram-positive rods (but few are Gram-negative)
- Endospores produced
- Aerobes or facultative anaerobes
- Growth on non-enriched media
- Most species motile, catalase-positive and
oxidase-negative
- Majority are non-pathogenic environmental
organisms
- Bacillus anthracis (non-motile) causes anthrax
- Bacillus licheniformis is implicated in sporadic abortions in cattle and sheep
- Usual habitat: environment

Answer 32

Bacillus species

Question 33

 Large, Gram-positive rods
 Endospores produced Anaerobic,  catalase-negative and oxidase- negative
 Motile (except C. perfringens)
 Enriched media required for growth
 Colonies of C. perfringens
surrounded by zones of double
haemolysis
 Present in soil, in alimentary tracts

Answer 33

clostridium species