Lesson 5b: Bacillus sp

Question 1

is implicated in sporadic abortions in cattle & ship

Answer 1

Bacillus licheniformis

Question 2

5 mm diameter, flat, dry, greyish
and with a ‘ground glass’ appearance after
incubation for 48 hours, curled outgrowths
from the edge of the colony impart a
characteristic, ‘medusa head’ appearance

Answer 2

B. anthracis

Question 3

same with B. anthracis but are larger
and with greenish tinge. Majority of strains
produce wide zone of complete haemolysis
around the colonies

Answer 3

B. cereus

Question 4

colonies are dull, rough, wrinkled
and strongly adherent to the agar that becomes
brown with age. Characteristic hair-like
outgrowths are produced from streaks of the
organisms on agar media

Answer 4

B. licheniformis

Question 5

which of these bacillus spieces are motile, haemolytic, resisitant to penicillin.

Answer 5

B. cereus

Question 6

this bacillus species is weak & slow in Lecithinase activity of egg yolk agar

Answer 6

B. anthracis

Question 7

this bacillus species are lysis in effect of gamma phage

Answer 7

B. anthracis

Question 8

this species if taken to pathogenecity for laboratory animal, dies within 24-28 hours

Answer 8

B. anthracis

Question 9

infectipon of this strain cause of abortion in cattle and sheep associated with the feeding of contaminated silage or mouldy hay and diagnosed by isolation in heavy, pure culture from foetal abomasal

Answer 9

B. licheniformis:

Question 10

this animals are highly suspectible to Anthrax

Answer 10

1. ruminant
2. pig
3. horses

Question 11

while these animals are resistant to anthrax

Answer 11

1. Carnivores
2. Birds ( due to their high body temperature)

Question 12

virulence factor of B. antracis is capsule and toxin both encoded by _______ and important in disease production

Answer 12

plasmid

Question 13

this plasmid PXO1 encodes for exotoxin which are;

Answer 13

1. protective antigen
2. oedema factor
3. lethal factor

Question 14

while this plasmid PXO2 encodes for capsule inhibiting ??

Answer 14

phagocytosis

Question 15

Clinical signs of Anthrax in cattle and sheep are usually ;

Answer 15

1. septicaemic
2. rapidly fatal
3. pyrexia w/ temperature up to 42°C (108°F)
4. depression
5. congested mucosae
6. petechiae observed in ante-mortem or subcu. oedema

Question 16

Clinical signs of antrax in pigs:

Answer 16

oedematous swelling of the throat and head along with regional lymphadenitis

Question 17

clinical signs of antrax in horses

Answer 17

extensive subcutaneous oedema of the thorax, abdomen or legs may develope swelling of the pharynx, colic and dysentery due to severe haemorrhagic enteritis may result from ingestion of spores

Question 18

in this animal clinical sign of antrax is, though rare, the course of the disease and pathological changes resemble those observed in affected pigs,

Answer 18

dog

Question 19

in diagnosis carcasses are seen:

Answer 19

1. Bloated
2. putrefy rapidly
3. do not exhibit rigor mortis
4. Dark, unclotted blood may issue from mouth & nostrills

Question 20

Direct blood smears using _______ from tail vein of ruminant or peritoneal fluid of pigs.

Answer 20

blood specimen

Question 21

Bacterial culture using;

Answer 21

Blood Agar or MacConkey Agar

Question 22

molecular test use:

Answer 22

PCR & Ascoli test

Question 23

Treatment and control:

Answer 23

: PenG or oxytet

Question 24

Control:

Answer 24

1. Vaccination
2. chemoprophylaxis
3. strict biosecurity measures
4. quarantine