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Lesson 8b: Escherichia coli Flashcards

motile w/ peritrichous flagella

1
Q

the cells of E.coli is composed of _____ that is used as antigens in serotyping E.coli;

A

lipopolysaccharide

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2
Q

this 3 are used for serotypic E.coli

A

Somatic (O)
Flgellar (H)
Capsular (k)

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3
Q

are protein in nature & the capsular antigens are composed of polysaccharide

A

Flagellar antigens

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4
Q

is present in many strains & act as adhesins facilitating attachment to mucosal surface

A

Proteinacious fimbrial (F) antigens

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5
Q

what are the predisposing factor thet permit colonization

A
  1. age
  2. immune status
  3. nature of diet
  4. heavy exposure to pathogenic strains
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6
Q

this interfere w/ the phagocytosis & with the antibacterial effectiveness of the complement system

A

capsule

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7
Q

released upon lysis or death of bacteria & endotoxin shock is a significant in septicaemia

A

endotoxin

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8
Q

endotoxin produce this _____ the lead to endothelial damage leading to disseminated intravascular coagulation

A

pyrogenic activity

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9
Q

this allows the attachement to mucosal surface & facilitate colonization by diminishing the expulsive effects of peristalsis & flushing of effect urine.

A

fimbrial adhesion

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10
Q

what is the adhesins of pigs that is present in E.coli

A

K88

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11
Q

adhesins of calves present in E.coli

A

F41

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12
Q

adhesins of calves & lambs present in E.coli

A

K99

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13
Q

what is the receptor found in older pigs, that is important in postweaning diarrhea & oedema disease

A

F18

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14
Q

this adhesins is associated in effacing E. coli that produce characteristics attaching & effacing lesion in which the bacteria are intimately adherent to the host epithelial cell membrane w/ marked rearrangement of the cytoskeleton

A

intimin

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15
Q

what are the 2 types of enterotoxin?

A
  1. heat labile (LT)
  2. heat stable (ST)
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16
Q

LT1 his is found in pigs, induces hypersecretion of fluid into the intestine tru stimulation of?

A

adenylate cyclase activity

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17
Q

LT2 is demonstrated in some ETEC strains isolated from ?

A

cattle

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18
Q

STa (heat stable a) is isolated from porcine, bovine, ovine & human speciemes. Toxins stimulate fluid & electrolyte secretions into small intestines & inhibits fluid absorption from the intestine by increasing?

A

intracellular guanosine monophosphate

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19
Q

in STb it causes secretion of this 2 ions & inhibit the absorption of this 1 ion

A
  1. chloride ion & bicarbonate ion
  2. sodium ion
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20
Q

produces heat-labile enterotoxin (LT) and/or heat-stable enterotoxin (ST). These toxins cause diarrheal illness by affecting the intestinal epithelial cells, leading to watery diarrhea.

A

Enterotoxigenic E.coli (ETEC)

21
Q

In ETEC this 3 can cause secretory diarrhea tru Chloride ion & bicarbonate ions secretion & inhibition of sodium ion absorption

A

LT, STa & STb

22
Q

They form characteristic “stacked-brick” biofilms on intestinal epithelial cells. Diarrhea in animals

A

Atypical (EAggEC)

23
Q

They form characteristic “stacked-brick” biofilms on intestinal epithelial cells. Diarrhea in humans

A

Typical EAggEC

24
Q

What are the 2 major toxins identify in Typical EAggEC

A
  1. East I
  2. Plasmid-encoded toxin
25
what is the major adhesins of E.coli producing oedema disease in pigs?
F18 fimbraie
26
this toxins in oedema in pigs contribute to disease damage & increase vascular permeability, that will lead to fluid accumulation & edema formation
Stx2e & alpha haemolysin
27
what are the 4 E.coli strains associated w/ extraintestinal disease
1. Avian pathogenic E. coli (APEC) 2. Strains causing localized infections 3. Septicaemic E. coli (SEPEC) 4. Europathogenic E. coli (UPEC)
28
what is the several adhesin of APEC?
1. adherence pili 2. long polar fimbrial 3. non-fimbrial adhesins
29
In APECS presence of this contributes to resistance to humoral immunity
Capsular antigens
29
what are the 2 toxin include in APEC
1. endotoxin 2. temperature-sensitive haemogglutinin
30
What is the virulence factor of APEC
Aerobactin iron uptake system
31
In Septicaemic E. coli what is the 2 adhesins that are found on the surface antigen in calves
1. fimbrial 2. CS3IA
32
What are the toxin produce in SEPEC?
1. endotoxin 2. CNF (cytotoxin necrotizing factor) 3. CDF ( cytolethal distending factor)
33
what is the major predisposing factor of SEPEC?
hypogammaglobulinemia
34
what is the resistance mechanism of SEPEC, that contribute to the resistance of ciruculating antigens?
1. capsular antigen 2. Smooth LPS
35
Europathogenic E. coli can cause ?
UTI & pyometra if left untreated
36
what is the major adhesins of UPEC?
type I, P & S fimbiraie
37
UPEC produces this 2 toxins
Alpha-haemolyisn & CNF-1
38
what are the suitable specimen in cases of pyometra or metritis?
midstream urine and cervical swabs
39
Specimens cultured on blood and MacConkey agar are incubated aerobically at
37°C for 24 to 48 hours
40
On blood agar, colonies are:
greyish, round and shiny with a characteristic smell, may be haemolytic or non-haemolytic
41
On MacConkey agar, colonies are;
bright pink
42
The colonies of some E. coli strains have a _____ _____on EMB agar.
Metallic sheen
43
______ _____ may be necessary to identify isolates from coliform mastitis or cystitis
Biochemical profile
44
When enterotoxigenic strains of E. coli are suspected, the presence of either enterotoxins or fimbrial antigens can be confirmed by:
immunological methods or molecular techniques such as PCR
45
Enterotoxins in the small intestine can be detected, using methods employing:
monoclonal antibodies.
46
For expression of fimbrial antigens, isolates should be subcultured on:
Minca medium
47
Fimbrial antigens can be identified using:
ELISA or latex agglutination.
48
PCR techniques using _____ ____ for genes encoding heat- labile and heat-stable enterotoxins may be used to identify enterotoxigenic strains of E. coli
primer specific

Bacteriology & Mycology (17 decks)

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