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Bacteriology & Mycology > Lesson 8b: Escherichia coli > Flashcards

Lesson 8b: Escherichia coli Flashcards

motile w/ peritrichous flagella

1
Q

the cells of E.coli is composed of _____ that is used as antigens in serotyping E.coli;

A

lipopolysaccharide

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2
Q

this 3 are used for serotypic E.coli

A

Somatic (O)
Flgellar (H)
Capsular (k)

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3
Q

are protein in nature & the capsular antigens are composed of polysaccharide

A

Flagellar antigens

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4
Q

is present in many strains & act as adhesins facilitating attachment to mucosal surface

A

Proteinacious fimbrial (F) antigens

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5
Q

what are the predisposing factor thet permit colonization

A
  1. age
  2. immune status
  3. nature of diet
  4. heavy exposure to pathogenic strains
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6
Q

this interfere w/ the phagocytosis & with the antibacterial effectiveness of the complement system

A

capsule

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7
Q

released upon lysis or death of bacteria & endotoxin shock is a significant in septicaemia

A

endotoxin

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8
Q

endotoxin produce this _____ the lead to endothelial damage leading to disseminated intravascular coagulation

A

pyrogenic activity

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9
Q

this allows the attachement to mucosal surface & facilitate colonization by diminishing the expulsive effects of peristalsis & flushing of effect urine.

A

fimbrial adhesion

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10
Q

what is the adhesins of pigs that is present in E.coli

A

K88

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11
Q

adhesins of calves present in E.coli

A

F41

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12
Q

adhesins of calves & lambs present in E.coli

A

K99

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13
Q

what is the receptor found in older pigs, that is important in postweaning diarrhea & oedema disease

A

F18

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14
Q

this adhesins is associated in effacing E. coli that produce characteristics attaching & effacing lesion in which the bacteria are intimately adherent to the host epithelial cell membrane w/ marked rearrangement of the cytoskeleton

A

intimin

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15
Q

what are the 2 types of enterotoxin?

A
  1. heat labile (LT)
  2. heat stable (ST)
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16
Q

LT1 his is found in pigs, induces hypersecretion of fluid into the intestine tru stimulation of?

A

adenylate cyclase activity

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17
Q

LT2 is demonstrated in some ETEC strains isolated from ?

A

cattle

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18
Q

STa (heat stable a) is isolated from porcine, bovine, ovine & human speciemes. Toxins stimulate fluid & electrolyte secretions into small intestines & inhibits fluid absorption from the intestine by increasing?

A

intracellular guanosine monophosphate

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19
Q

in STb it causes secretion of this 2 ions & inhibit the absorption of this 1 ion

A
  1. chloride ion & bicarbonate ion
  2. sodium ion
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20
Q

produces heat-labile enterotoxin (LT) and/or heat-stable enterotoxin (ST). These toxins cause diarrheal illness by affecting the intestinal epithelial cells, leading to watery diarrhea.

A

Enterotoxigenic E.coli (ETEC)

21
Q

In ETEC this 3 can cause secretory diarrhea tru Chloride ion & bicarbonate ions secretion & inhibition of sodium ion absorption

A

LT, STa & STb

22
Q

They form characteristic “stacked-brick” biofilms on intestinal epithelial cells. Diarrhea in animals

A

Atypical (EAggEC)

23
Q

They form characteristic “stacked-brick” biofilms on intestinal epithelial cells. Diarrhea in humans

A

Typical EAggEC

24
Q

What are the 2 major toxins identify in Typical EAggEC

A
  1. East I
  2. Plasmid-encoded toxin
25
Q

what is the major adhesins of E.coli producing oedema disease in pigs?

A

F18 fimbraie

26
Q

this toxins in oedema in pigs contribute to disease damage & increase vascular permeability, that will lead to fluid accumulation & edema formation

A

Stx2e & alpha haemolysin

27
Q

what are the 4 E.coli strains associated w/ extraintestinal disease

A
  1. Avian pathogenic E. coli (APEC)
  2. Strains causing localized infections
  3. Septicaemic E. coli (SEPEC)
  4. Europathogenic E. coli (UPEC)
28
Q

what is the several adhesin of APEC?

A
  1. adherence pili
  2. long polar fimbrial
  3. non-fimbrial adhesins
29
Q

In APECS presence of this contributes to resistance to humoral immunity

A

Capsular antigens

29
Q

what are the 2 toxin include in APEC

A
  1. endotoxin
  2. temperature-sensitive haemogglutinin
30
Q

What is the virulence factor of APEC

A

Aerobactin iron uptake system

31
Q

In Septicaemic E. coli what is the 2 adhesins that are found on the surface antigen in calves

A
  1. fimbrial
  2. CS3IA
32
Q

What are the toxin produce in SEPEC?

A
  1. endotoxin
  2. CNF (cytotoxin necrotizing factor)
  3. CDF ( cytolethal distending factor)
33
Q

what is the major predisposing factor of SEPEC?

A

hypogammaglobulinemia

34
Q

what is the resistance mechanism of SEPEC, that contribute to the resistance of ciruculating antigens?

A
  1. capsular antigen
  2. Smooth LPS
35
Q

Europathogenic E. coli can cause ?

A

UTI & pyometra if left untreated

36
Q

what is the major adhesins of UPEC?

A

type I, P & S fimbiraie

37
Q

UPEC produces this 2 toxins

A

Alpha-haemolyisn & CNF-1

38
Q

what are the suitable specimen in cases of pyometra or metritis?

A

midstream urine and cervical swabs

39
Q

Specimens cultured on blood and MacConkey agar are incubated aerobically at

A

37°C for 24 to 48 hours

40
Q

On blood agar, colonies are:

A

greyish, round and shiny with a characteristic smell, may be haemolytic or non-haemolytic

41
Q

On MacConkey agar, colonies are;

A

bright pink

42
Q

The colonies of some E. coli strains have a _____ _____on EMB agar.

A

Metallic sheen

43
Q

______ _____ may be necessary to identify isolates from coliform mastitis or cystitis

A

Biochemical profile

44
Q

When enterotoxigenic strains of E. coli are suspected, the presence of either
enterotoxins or fimbrial antigens can be confirmed by:

A

immunological methods or molecular techniques such as PCR

45
Q

Enterotoxins in the small intestine can be detected, using methods employing:

A

monoclonal antibodies.

46
Q

For expression of fimbrial antigens, isolates should be subcultured on:

A

Minca medium

47
Q

Fimbrial antigens can be identified using:

A

ELISA or latex agglutination.

48
Q

PCR techniques using _____ ____ for genes encoding heat- labile and heat-stable enterotoxins may be used to identify enterotoxigenic strains of E. coli

A

primer specific

Bacteriology & Mycology (17 decks)

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