Lesson 4a: Corynebacterium species

Question 1

• Gram-positive, pleomorphic bacteria
• Fastidious, requiring enriched media

Answer 1

Corynebacterium species

Question 2

usual habitat of corynebacterium :

Answer 2

mucous membrane

Question 3

Identification criteria of corynebacterium

Answer 3

o Morphology
o Colonial characteristics
o Biochemical react

Question 4

most species of corynebacterium is oppotunistics except;

Answer 4

C. bovis

Question 5

is a facultative intracellular pathogen capable of surviving and replicating in phagocytes whose virulence is linked to its cell wall lipid and to the production of an exotoxin, phospholipase D (PLD) that enhances its survival and multiplication in the host

Answer 5

C. pseudotuberculosis

Question 6

Diptheria toxin from 2 species present in raw milk cows may implicate public health, what are the 2 species ?

Answer 6

C. ulcerans and C. pseudotuberculosis

Question 7

in diagnosis Isolation and identification of bacteria from cell culture on blood agar, selective blood agar and MacConkey agar at how many degree & days ?

Answer 7

37°C for 1 to 2 days

Question 8

Identification criteria for isolate

Answer 8

• Colonial characteristics
• Morphology in stained smears
• Presence or absence of haemolysis on blood agar
• Absence of growth on MacConkey agar
• Enhancement of haemolysis test for C. pseudotuberculosis

Question 9

Molecular detection through

Answer 9

PCR

Question 10

Caseous lymphadenits is caused by ______ in goats, sheep and cattle.

Answer 10

non-nitrate-reducing biotype of C. pseudotuberculosis

Question 11

Manifestations of caseous lymphadenitis:

Answer 11

1. chronic suppurative conditions
2. encapsulated abscess formation “have an onion appearance”
3. enlargement of lymph nodes

Question 12

Corynebacterium pseudotuberculosis has been isolated from the _____ of affected goats thus posing a threat to public

Answer 12

milk

Question 13

Sheep become infected by caseous lymphadenitis through ___________ by arthropod bites or from contaminated dips.

Answer 13

Contamination of shearing wound

Question 14

Dx of caseous lymphadenitis:

Answer 14

1. clinical lesions
2. Gram-staining
3. Bacterial culture from abscess swab samples, or detection antibodies by ELISA

Question 15

Tx of caseous lymphadenitis

Answer 15

Tx: not effective,
Tx of control: strict border control, heightened
biosecurity, or culling

Question 16

caused by the nitrate-reducing biotype of C. pseudotuberculosis in horses and cattle. Prevalent in countries with temperate weather

Answer 16

Ulcerative lymphadenitis

Question 17

caused by C. renale group. The stress of parturition and the short urethra in the cow predispose to infection of the urinary tract.

Answer 17

Bovine pyelonephritis

Question 18

Clinical signs of bovine pyelonephritis include:

Answer 18

1. Fever
2. anorexia
3. decrease milk production

Question 19

Dx of bovine pyelonephritis:

Answer 19

1. Rectal palpation (unilateral thickened ureter/enlarged kidneys)
2. bacterial culture from urine sample
3. Presence of rbc or protein in urine

Question 20

Tx of bovine pyelonephritis:

Answer 20

Penicillin

Question 21

also called pizzle rot common in Merino sheep and Angora goats, is caused by C. renale and is characterized by ulceration around the preputial orifice (or vulva in ewes), with a brownish crust developing over the lesion

Answer 21

Ulcerative balanoposthitis

Question 22

In non-nitrate reducing biotype of C. pseudotubercolusis the sheep & goats has this disease condition.

Answer 22

Caseous lymphadenitis

Question 23

in C. pseudotubercolusis the nitrate-reducing biotype the horses & cattle has this disease.

Answer 23

Ulcerative lymphagitis, abscess

Question 24

corynebacterium sp. occurs singly in?

Answer 24

palisades of parallel cells

Question 25

in angular cluster, corynebacterium sp. it resembles?

Answer 25

Chinese characteristics in stained smears

Question 26

C. pseudotubercolosis produces this 2 to enhances survival & multiplication.

Answer 26

1. eoxotoxin
2. phospholipase d