Lesson 3b: Nocardia species

Question 1

Branching filaments of ______ have a tendency to fragment.

Answer 1

Nocardia asteroids

Question 2

what is the usual mode of infection of Nocardia sp.

Answer 2

1. By inhalation
2. Skin wounds
3. Teat canal

Question 3

an intestinal form of ______ may result from ingestion of the organisms.

Answer 3

Nocardiosis

Question 4

Is a virulent strain of nocardia species, that the productions of superoxide dismutase & catalase & presence of thick peptidoglycan layer in cell wall confer resistance to microbial activity of phagocytes.

Answer 4

N. asteroides

Question 5

To reveal the clusters of nocardial filaments what are the specimen suitable for laboratory examination?

Answer 5

1. Exudates
2. Aspirates
3. Mastitic milk
4. Tissue from granulomas
5. Fixed tissue of histopathology

Question 6

methods used in Nocardia species:

Answer 6

1. Gram-staining
2. MZN methods

Question 7

Bacterial culture on Nocardia species:

Answer 7

1. Blood agar
2. Selective growth enhancing media such as ( CHARCOAL)

Question 8

charcoal is a?

Answer 8

yeast extract medium

Question 9

colonies on blood agar are usually visible after incubation for about how many days? and what characteristics are seen?

Answer 9

5 days
1. White
2. Powdery
3. Firmly adherent to the agar
4. Colonies are haemolytic & odourless

Question 10

Subculture on to Sabouraud dextrose agar yields this ______ after incubation for up to 5days

Answer 10

1. yields dry
2. wrinkled
3. oranged-coloured colonies

Question 11

Gram-stained smears from colonies show some ?

Answer 11

Filamentous forms w/ a preponderance of rod & coccal forms

Question 12

DNA sequencing of this _____ is frequently used method for identification of nocardial species.

Answer 12

16s rRNA

Question 13

Nocardia sp. infection tx is:

Answer 13

1. Amikacin
2. Imipenem-cilastatin
3. Cotrimoxazole

Question 14

Nocardia species in dog causes what kind of disease?

Answer 14

Canine nocardiosis
-cutaneous pyogranulomas
-pyogranulomatous pleural lesions & pyothorax
-disseminated lesions

Question 15

Nocardia species in cattle causes?

Answer 15

1. Chronic mastitis
2. Abortion bovine farcy

Question 16

Nocardia species in pigs causes?

Answer 16

Abortion

Question 17

Nocardia species in sheep, goats horses causes?

Answer 17

1. wound infection
2. mastitis
3. pneumonia
4. other pyogranulomatous conditiond.