Lesson 8c: Salmonella serotypes

Question 1

this identify O & H antigens, and occasionaly Vi antigens. It’s used in serotyping salmonella bacteria

Answer 1

Kaufmann & white

Question 2

the majority of salmonella belong to what?

Answer 2

S. eneterica subspecies eneterica

Question 3

developed for a small numbers of serotypes & used for epidemiological investigations of isolates

Answer 3

Phage typing

Question 4

what is the main route of salmonella?

Answer 4

Ingestion

Question 5

route can also be in?

Answer 5

Upper respiratory tract & Conjuctiva

Question 6

in pigs, salmonella colonize & persist in?

Answer 6

Tonsils

Question 7

2 Virulence factor of Salmonella serotypes:

Answer 7

1. Fimbriae (type 1) & long polar fimbriae
2. Protein that interact w/ actin cytoskeleton of hos cells

Question 8

In case of salmonella entereditis, it infect where?

Answer 8

ovaries

Question 9

this enables to adhere to intestinal mucosae (peyers patches)

Answer 9

Type 1 Fimbriae

Question 10

this can cause rearrangements that leads to ruffle formation

Answer 10

protein that interact w/ actin cytoskeleton of the host cells.

Question 11

Salmonella localized the mucous lining of the ?

Answer 11

1. ileum
2. cecum
3. colon
4. mesenteric lymph nodes

Question 12

if pathogens evade clearance mechanism this 2 may occur:

Answer 12

1. subclinical infection
2. latent infection

Question 13

this indicates low number of slamonella in the feces without causing obvious symptoms

Answer 13

Subclinical infection

Question 14

when salmonella can remain dormant on places like gallbladder or mesenteric lymph nodes without being secreted.

Answer 14

Latent infection

Question 15

this clinical may developed due to predisposing factors.

Answer 15

1. stress
2. amount of salmonella ingested
3. virulence of specific salmonella or strains
4. suspectibility of host to combat infection

Question 16

this affects farm animals, regardless of age

Answer 16

Enteric salmonellosis

Question 17

If enteric salmonellosis manifest as acute, what are the possible symptoms?

Answer 17

1. fever
2. depression
3. loss of appetite
4. severe diarrhea w/ blood & mucous
5. epithelial casts

Question 18

If enteric salmonellosis may developed into chronic eneterocolitis, what are possible symptoms ?

Answer 18

1. intermittent fever
2. soft feces
3. gradual weight loss that leads to emaciation

Question 19

it affects calves, neonatal foals & pigs younger than 4 months

Answer 19

Septicaemic salmonellosis

Question 20

pigs affected to this, has distinguished discoloration of ears & snout

Answer 20

Salmonell cholerasuis

Question 21

Salmonella pullorum, is called ____.

Answer 21

Pullorum disease or Bacillary white diarrhea

Question 22

chicks & turkey affected by S. pullorum has how many weeks w/ high mortality rate?

Answer 22

2-3 ,weeks

Question 23

Fowl thypoid produced lesion similar to S. pullorum, it’s caused by ____,

Answer 23

S. gallinarium

Question 24

this name is given to infection of poultry by non-host adapted salmonellae, such as entereditis & Typhimurim.

Answer 24

Parathyroid caused by S. entereditis

Question 25

if isolation of salmonella from blood or parenchymatous organs it's?

Answer 25

confirmatory

Question 26

if there is heavy growth of S. from feces, it suggest?

Answer 26

pathogen involved

Question 27

in recovering small numbers of salmonellae is present in the feces it indicated?

Answer 27

Carrier state

Question 28

what are the 2 selective media used in salmonella for colony characteristics ?

Answer 28

1. Brilliant green agar 2. Xylose lysine deoxycholate

Question 29

in this selective media, S. colonies & medium appear ?

Answer 29

red in alkilinity

Question 30

in this selective media, S. colonies are _____ indicating H2O production

Answer 30

Red w/ Black

Question 31

In case of biochemical testing, if suspicious colonies from selective media are subcultured onto?

Answer 31

1. Triple sugar Iron (TSI) Agar 2. lysine decarboxylase broth

Question 32

how many hours & temperature for biochemical test are conducted to established the identity of salmonella?

Answer 32

18 hours at 37 degree celcius

Question 33

What is the molecular technique used in S. serotypes, for repid & specific detection of salmonella?

Answer 33

PCR & real time PCR

Question 34

What is serological test used in S. serotypes for diagnosing herd & flock

Answer 34

ELISA & Agglutination technique

Question 35

what can be used for sample collection:

Answer 35

1. Tissue 2. Feces

Question 36

in fecal sample, note that it's first ***inoculated*** into _____, incubated at 37 degree celsius for 48 hours aerobically

Answer 36

Enrichement broth (selenite F, Rappaport or Tetrathionate)

Question 37

after incubation of fecal sample, the enriched broth is again ***subcultured*** onto _____, this helps in the isolation & identification of salmonella

Answer 37

selective blood agar media (MacConkey, Brilliant agar or XLD )

Question 38

In tissue sampling it's directed to inocultaion on _________, & then incubted at 37 degree celsius at 24 hours

Answer 38

Selective blood agar