Lesson 8c: Salmonella serotypes Flashcards
The virulence of Salmonella serotypes relates to their ability to invade and replicate in epithelial cells which are encoded on Salmonella pathogenicity islands (SPI) and virulence plasmid
this identify O & H antigens, and occasionaly Vi antigens. It’s used in serotyping salmonella bacteria
Kaufmann & white
the majority of salmonella belong to what?
S. eneterica subspecies eneterica
developed for a small numbers of serotypes & used for epidemiological investigations of isolates
Phage typing
what is the main route of salmonella?
Ingestion
route can also be in?
Upper respiratory tract & Conjuctiva
in pigs, salmonella colonize & persist in?
Tonsils
2 Virulence factor of Salmonella serotypes:
- Fimbriae (type 1) & long polar fimbriae
- Protein that interact w/ actin cytoskeleton of hos cells
In case of salmonella entereditis, it infect where?
ovaries
this enables to adhere to intestinal mucosae (peyers patches)
Type 1 Fimbriae
this can cause rearrangements that leads to ruffle formation
protein that interact w/ actin cytoskeleton of the host cells.
Salmonella localized the mucous lining of the ?
- ileum
- cecum
- colon
- mesenteric lymph nodes
if pathogens evade clearance mechanism this 2 may occur:
- subclinical infection
- latent infection
this indicates low number of slamonella in the feces without causing obvious symptoms
Subclinical infection
when salmonella can remain dormant on places like gallbladder or mesenteric lymph nodes without being secreted.
Latent infection
this clinical may developed due to predisposing factors.
- stress
- amount of salmonella ingested
- virulence of specific salmonella or strains
- suspectibility of host to combat infection
this affects farm animals, regardless of age
Enteric salmonellosis
If enteric salmonellosis manifest as acute, what are the possible symptoms?
- fever
- depression
- loss of appetite
- severe diarrhea w/ blood & mucous
- epithelial casts
If enteric salmonellosis may developed into chronic eneterocolitis, what are possible symptoms ?
- intermittent fever
- soft feces
- gradual weight loss that leads to emaciation
it affects calves, neonatal foals & pigs younger than 4 months
Septicaemic salmonellosis
pigs affected to this, has distinguished discoloration of ears & snout
Salmonell cholerasuis
Salmonella pullorum, is called ____.
Pullorum disease or Bacillary white diarrhea
chicks & turkey affected by S. pullorum has how many weeks w/ high mortality rate?
2-3 ,weeks
Fowl thypoid produced lesion similar to S. pullorum, it’s caused by ____,
S. gallinarium
this name is given to infection of poultry by non-host adapted salmonellae, such as entereditis & Typhimurim.
Parathyroid caused by S. entereditis
if isolation of salmonella from blood or parenchymatous organs it’s?
confirmatory
if there is heavy growth of S. from feces, it suggest?
pathogen involved
in recovering small numbers of salmonellae is present in the feces it indicated?
Carrier state
what are the 2 selective media used in salmonella for colony characteristics ?
- Brilliant green agar
- Xylose lysine deoxycholate
in this selective media, S. colonies & medium appear ?
red in alkilinity
in this selective media, S. colonies are _____ indicating H2O production
Red w/ Black
In case of biochemical testing, if suspicious colonies from selective media are subcultured onto?
- Triple sugar Iron (TSI) Agar
- lysine decarboxylase broth
how many hours & temperature for biochemical test are conducted to established the identity of salmonella?
18 hours at 37 degree celcius
What is the molecular technique used in S. serotypes, for repid & specific detection of salmonella?
PCR & real time PCR
What is serological test used in S. serotypes for diagnosing herd & flock
ELISA & Agglutination technique
what can be used for sample collection:
- Tissue
- Feces
in fecal sample, note that it’s first inoculated into _____, incubated at 37 degree celsius for 48 hours aerobically
Enrichement broth
(selenite F, Rappaport or Tetrathionate)
after incubation of fecal sample, the enriched broth is again subcultured onto _____, this helps in the isolation & identification of salmonella
selective blood agar media
(MacConkey, Brilliant agar or XLD )
In tissue sampling it’s directed to inocultaion on _________, & then incubted at 37 degree celsius at 24 hours
Selective blood agar