Lesson 2a; staphylococcus species Flashcards
in characteristic ‘bunches of grapes’ formations
STAPHYLOCOCCI
staphylococcus a. in cattle cause what?
mastitis
s. hyicus in cattle cause what?
- mastitis
s. aureus subsp. anaerobius in sheep causes what?
lymphadenitis
pyogenic characteristics causes what?
suppurative lesions
staphylococcal cell wall protein, which binds to this 2 ________ facilitate bacterial attachments to tissues
fibronectin & fibrinogen
what are the 3 structural features that interefere with opsonization & subsequent phagocytosis
- polysaccharide
- teichoic acids
- protein a
_____ this production aids in survival within phagocytosis.
catalase production
_____ has a role in shielding the organism from phagocytic cells
coagulase
what are the 2 exoenzymes that promote tissue invasion
kinases & hyaluronidase
staphylococcal disease of importance in domestic animals include 5 diseases, what are those? remember: pymatiboex
- pyoderma
- mastitis
- tick pyaemia
- bortryomycosis
- exudative epidermitis
an s. aureus disease, most often causes subclinical infection; virulence factor is alpha toxin that result to tissue necrosis & can be life threatening. What type of disease has this clinical infections?
BOVINE STAPHYLOCOCCAL MASTITIS
what is the diagnosis & treatment of anaplasma phagocytophilum ?
dx. Bacterial confirmations
tx. tatracyclines & tick control
s. aureus in horses; a chronic, suppurative granulomatous that occur within a few weeks after castration due to infection of the stump of the spermatic cord; can also occur in mammary tissues of sows. What kind of disease is this.
Botryomycosis
dogs with wound infections, surgical site infections, pyoderma, otitis & urinary tract infections are most commonly reported; common cause of nasocomial infection in vet hospitals.
Methicillin-resistant staphylococcal infections in animals
In lambs they carry S. aureus in what location of their body?
- Skin
- Nasal mucosa
- Infection occurs through minor skin trauma
what are the 2 microorganisms that can cause tick pyaemia:
- Ixodes ricinus
- Anaplasma phagocytophilum
is a vector for the rickettsial agent of tick-borne fever
Ixodes ricinus
this can cause immuno suppression in lambs & may predispose to staphylococcal infection.
Anaplasma phagocytophilum
Tick pyaemia is characterized by either :
- septicaemia
- rapid death
- localized abscess
what is the diagnosis used in tick pyaemia?
Bacterial confirmations
what can be the treatment for tick pyaemia?
- Tetracyclines
- Tick control
S. hyicus occurs in sucklers & weaned pig, it’s highly contagious, & can be isolated from the vaginal mucosa & skin of healthy sows.
Exudative epidermitis (Greasy pig disease)
S. hyicus of exudative epidermitis enter to this location:
Skin of young pigs through minor abrasions as bite wounds
Exudative dermatitis can be characterized by:
- Greasy exudate
- Exfoliation
- Exudation on skin.
is the major virulence factor of exudative epidermitis as injection of this toxin into the skin of young pigs produces exfoliation?
Exfoliative toxin
2 dx of exudative epidermitis
- Isolation
- identification of bacteria from dermal lesions
tx of S. hyicus in exudative epidermitis
Antibacterial therapy
what are the lesions of staphylococcus species:
- Skin scrapping
- Swab sample
what is the exudates in staphylococcus species:
- Mastitis milk
What can be used in the demonstration of bacteria:
- Gram staining
- Culture on blood agar
- Selective blood agar
- MacConkey agar
6 identification criteria use in staphylococcus species;
- Colonial characteristics
- Presence or Absence of haemolysis
- Absence of growth on MacConkey agar
- Catalase production
- Coagulase production
- Biochemical profile
molecular typing use here is ?
PCR procedures
this 2 differs in all Staphylococcus species because they are anaerobic & catalase-negative
- S. aureus subsp. anaerobius
- S. saccharolyticus
Colonies of this 2 produce double haemolysis:
- S. aureus
- S. pseudointermedius
2 exotoxins lyse host cell membranes.
- haemolysis
- leukocidin
