Week 4 notes Flashcards
What are the body fluids we look at in cytology?
CSF, peritoneal fluid, pleural fluid, synovial fluid and blood
Histology is examining what?
a slice of tissue
Cytology is examining what?
exfoliated cells
What are some of the collections we take?
swabs, vaginal smear, scraping, FNB, FNA, biopsies, imprints, centesis, wash/flush, semen
What are the different biopsies we do?
wedge and punch
How do you do a swab smear?
roll it gently on the slide
how do you do a compression smear (squash prep)?
put a dot of (?) on to a slide and then put another slide over top that to smoosh it and then they are slid apart or you turn the second slide.
how do you do a combination technique smear?
blood smear on one side compression on the other and a middle part that is untouched.
how do you do a line smear?
similar to blood smear but you lift early.
how long should a slide stay in the fixative for cytology?
5 minute minimum, longer is better
When submitting samples to a reference lab what needs to be on the label?
signalment, brief history, relevant examination data, previous therapy/test results, differential diagnosis
What information about the sample do we put down?
site it came from, mass description
how do you fix a tissues sample for a histopathologic evaluation?
in 10% formalin
What does TNCC stand for?
total nucleated cell count
How is inflammation identified?
presence of WBCs; neutrophils, and macrophages, maybe eosinophils or lymphocytes, TNCC of 5,000+ cells/mcL, fluid is often turbid and white or pale yellow, TP is often 3+g/dL
