Virus Classification, structure, and replication

Question 1

How is a Virus different than a bacterium?

Answer 1

Size-Smaller

Growth-

Question 2

Two theories of virus origin.

Answer 2

Cellular origin-Once cellular components but evolved separately.
Autopoietic origin-Like mitochondria-once self sustaining, became dependent on cells for replication

Question 3

Attributes for Virus classification. (5)

Answer 3

Particle Structure,
Genome,
Replication features,
Serology,
Stability

Question 4

RNA or DNA located in___

Answer 4

Core

Question 5

Outer layer protein coat that protects DNA/RNA

Answer 5

Capsid

Question 6

What advantages/Disadvantages come with a large genome?

Answer 6

More proteins/more chance for error, able to be detected

Question 7

Step of replication where all the parts of viruses are put together .

Answer 7

Assembly

Question 8

Lystic infections

Answer 8

Lysis kills the cell

Question 9

Non-lytic infections

Answer 9

Don’t actually kill the cell, can be continually shed from the cell

Question 10

Detection of viruses

Answer 10

Discovery,
Confirmation,
Principle detection and quantification

Question 11

Cytopathic effect

Answer 11

Kills the cell:

Cell rounding, Syncytia formation, Inclusion bodies

Question 12

Fluorescent focus assay

Answer 12

Infect cells, expose antigen, stain with labeled antibody- count fluorescence

Question 13

Plaque Assay

Answer 13

Plaques show up, each spot represents a virus particle

Question 14

Infectious dose assays

Answer 14

Eggs/animals, find out at what dose do 50% die

Question 15

Particle assays (2)

Answer 15

Electron microscopy,

Hemaglutinin asssay