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stuff ni jas > uv-vis lmao > Flashcards

uv-vis lmao Flashcards

1
Q

Light is a form of what?

A

electromagnetic radiation

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2
Q

how is electromagnetic radiation transmitted

A

through waves

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3
Q

what is wavelength

A

distance between maxima and minima of waves

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4
Q

what is frequency

A

number of oscillations of the field per second

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5
Q

what are distinct particles of electromagnetic waves called

A

photons

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6
Q

true or false: energy inversely proportional to wavelength

A

true

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7
Q

what is the wavelength range for visible light

A

380nm to 740nm

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8
Q

what is the perception when observed color is being selectively absorbed by chemical species

A

complimentary colors

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9
Q

when a photon of colored light is absorbed by a compound what happens?

A

electrons transition from lower orbital energy (ground state) to higher energy orbital (excited state)

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10
Q

what is molar absorptivity?

A

measure of how strongly the solution absorbs light in a wavelength

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11
Q

how long usually is the pathlength?

A

1cm

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12
Q

what does a spectrometer do?

A

produces a desired range of wavelength of light

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13
Q

what is a collimator?

A

it is a lens that transmits a straight beam of light (photons)

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14
Q

what is a monochromator?

A

it is a prism that splits the beams into several component wavelengths (spectrum)

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15
Q

what is a wavelength selector?

A

transmits only the desired wavelengths

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16
Q

what is the light source for visible regions?

A

tungsten lamp

17
Q

what is the light source for ultraviolet regions?

A

deuterium

18
Q

what does a photometer do?

A

detects the amount of photons absorbed and sends a signal to a galvanometer or digital display

19
Q

what does a uv-vis spectrophotometer do?

A

makes the sample absorb light in the visible and near ultraviolet region of the electromagnetic spectrum

20
Q

where are spectrophotometers usually used?

A

colorimetric assays to measure the amount of light transmitted through a sample at a given wavelength

stuff ni jas (20 decks)

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