chemical tests for proteins Flashcards

1
Q

(+) for peptide bonds

A

biuret test

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2
Q

red coloration

A

nitroprusside test

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3
Q

reagents of conc. HNO3 and conc. NaOH

A

xanthoproteic test

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4
Q

(+) for phenolic group of tyrosine

A

millon’s test

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5
Q

red to red-orange color

A

sakaguchi test

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6
Q

reagents of NaOH and CuSO4

A

biuret test

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7
Q

(+) for the indole group of tryptophan

A

hopkins cole test

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8
Q

(+) for methionine and cysteine

A

fohl’s test

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9
Q

(+) for guanidino group of arginine

A

sakaguchi test

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10
Q

reagent is 1,2,3-indanetrione monohydrate / triketohydrindene with EtOH

A

ninhydrin test

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11
Q

(+) for cysteine

A

nitroprusside test

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12
Q

(+) for histidine and tyrosine

A

pauly’s test

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13
Q

reagent is lead acetate

A

fohl’s test

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14
Q

blue to blue-purple or yellow-orange product

A

ninhydrin test

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15
Q

(+) for proline in orsnge residue

A

ninhydrin test

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16
Q

pink to violet to blue coloration

A

biuret test

17
Q

principle is complexation thru mercuration & nitration or nitrosation/ complexation of nitrohydroxyphenyl derivatives with Hg2+

A

millons test

18
Q

(+) for F, Y, W aromatic AAs

A

xanthoproteic test

19
Q

(+) for free alpha amino acids and amines

A

ninhydrin test

20
Q

principle is complexation of copper with a peptide bond

A

biuret test

21
Q

reagents are alpha naphthol, NaOH, NaOBr, urea

A

sakaguchi test

22
Q

principle is diazotized sulfanilic acid with amino phenol to form a red azor in cold conditions

A

pauly’s test

23
Q

reagent is Na2Fe(CN)5NO in dilute NH3

A

nitroprusside test

24
Q

old rose/ flesh/ purple-red ppt.

A

millons test

25
Q

yellow solution with heating that has orange excess NaOH

A

xanthoproteic test

26
Q

pink to violet interface

A

hopkins cole test

27
Q

nitration of aromatic rings

A

xanthoproteic test

28
Q

reagents are glyoxylic acid and conc. H2SO4

A

hopkins cole test

29
Q

brown to black ppt.

A

fohl’s test

30
Q

principle is complexation

A

nitroprusside test

31
Q

detects 1,2,3 amines

A

amide testr

32
Q

base-catalyzed condensation of alpha naphthol with guanidino group of arginine

A

sakaguchi test

33
Q

oxalate to glyoxylate and condensation of 2 tryptophans with glyoxylic acid

A

hopkins cole

34
Q

oxidative decarboxylation and deamination followed by condensation

A

ninhydrin test

35
Q

degradation and substitution to form PbS

A

fohl’s test

36
Q

reagent is NaOH

A

amide test

37
Q

turns red litmus to blue

A

amide test

38
Q

base-catalyzed hydrolysis

A

amide test