Urinalysis - Macroscopic Examination Flashcards
Urine Average Daily Output
1200 - 1500 ml
600-2000 ml
Major ORGANIC Component
Urea
Major INORGANIC Component
Chloride
Routine Analysis - Specimen Collection
Capacity: ______
Required Volume: _______
Average Volume: _______
Specimen Collection
Routine Urinalysis - Capacity: 50 mL
Required Volume: 10 - 15 mL
Average Volume: 12 mL
Drug Analysis - Specimen Collection
Capacity: ______
Required Volume: _______
Average Volume: ______
Temperature: ______
Adulterant: ______
Drug Analysis - Specimen Collection
Capacity: 60 mL
Required Volume: 30 - 45 mL
Average Volume: 37.5 mL
Temperature: 32.5 - 37.7 C; checked within 4 minutes
Adulterant: Water, Methylene Blue
After collection specimen should be delivered and processed within ____ hours
2 hours
Increased - Changes in Unpreserved Urine
Mnemonics: phaBaON
pH
Bacteria
Odor
Nitrite
*Darkened Color
Least affected analyte in Unpreserved urine
Protein
Routine Method of Urine preservation
Refrigeration (2C - 8C)
Preserves Protein, and used for Culture & Sensitivity
Boric Acid
Best Sediment preservative and used for Addis Count
Formalin
Preservative that prevent glycolysis and used for Drug Analysis
Sodium Fluoride
Preservative in Cytology Studies
Saccomano Fixative
Specimen in Routine Urinalysis
Random Urine Specimen
Ideal Specimen for Routine UA & Pregnancy Test, Crystal identification
First Morning Urine
Used for Evaluation for Orthostatic Proteinuria
First Morning Urine
Most sterile specimen
Suprapubic Needle Aspiration
Sterile Specimens used for Culture
Midstream Clean Catch
Catheterized
Suprapubic
Specimen for Addis Count
12 hr Urine Specimen
Specimen for Urobilinogen
2 hr specimen (2pm - 4pm)
Specimen for Nitrite
4 hr specimen
Specimen for Glucose or Diabetic monitoring
2nd morning or Fasting
3 Glass Collection
Prostatitis specimen
Negative Control for Prostatitis specimen
Tube 2
Interpret:
Tube 2 - High Bacteria
UTI
Invalid Test
Interpret:
Tube 3 - 10x higher bacteria than Tube 1
Prostatic Infection
Major Urine Pigment
Urochrome
Oxidation product of Urobilinogen
Urobilin
Pink pigment
Uroerythrin
Dar Yellow Urine
Concentrated
Dehydration
Orange Urine with Yellow Foam/Tea colored urine
Bilirubin
Orange Urine (viscous) with Orange Foam
Phenazopyridine
Portwine Urine
Porphyrins
Green Urine
P. aeroginosa
Phenol
Black Urine
Methemoglobin (Acidic)
Melanin (exposed to air exposure)
Homogentisic Acid (Alkaline)
Cloudy Red Urine
Intact RBC
Clear Red Urine
Hemoglobin
Normal Urine Odor
Aromatic
Odorless
Acute Tubular Necrosis
Caramelized sugar, curry, maple syrup
MSUD
Ammoniacal/Pungent
Old Urine , UTI
Mousy/musty
Phenylketonuria
Rancid Odor
Tyrosinemia
Cabbage/hops
Methionine malabsorption
Sweaty feet/Acrid:
Isovaleric/Glutaric acidemia
Bleach
Contamination
No visible particles
Clear
Hazy
Few particulates, Print easily seen
Many particulates, Print turbid
Cloudy
Turbid
Print cannot be seen
Milky
Precipitate/Clotted
Correlations of Urine Turbidity
Acidic Urine:
Alkaline Urine:
Acidic Urine: Amorphous Urates, Radiographic Contrast Media
Alkaline Urine: Amorphous phosphates, Carbonates
Correlations of Urine Turbidity - Soluble in Heat
Amorphous Urates
Uric Acid Crystal
Correlations of Urine Turbidity
Soluble in Dilute acetic Acid:
Insoluble in Dilute Acetic Acid:
Correlations of Urine Turbidity
Soluble in Dilute acetic Acid: RBCs, Amorphous phosphates, Carbonates
Insoluble in Dilute Acetic Acid: WBCs, Yeast, Spermatozoa
Specific Gravity of the plasma filtrate entering the glomerulus
1.010
Specific Gravity
Normal Random SG:
Common Random SG:
Not Urine SG:
Specific Gravity
Normal Random SG: 1.002 - 1.035
Common Random SG: 1.015 - 1.030
Not Urine SG: < 1.002
Water Specific Gravity
1.000
Requires Temperature Correction and Glucose, Protein Correction
Urinometry
Urinometry
Volume Required:
Calibrator Temperature;
Calibrator:
Urinometry
Volume Required: 10 - 15 mL
Calibrator Temperature: 20C
Calibrator: Potassium Sulfate (K2SO4)
Temperature Corrections
> 20C:
< 20C:
Temperature Corrections
> 20C: + 0.001 every 3C
< 20C: - 0.001 every 3C
Glucose and Protein Correction
1 g/dL Glucose: ______
1 g/dL Protein: ______
Glucose and Protein Correction
1 g/dL Glucose: - 0.004
1 g/dL Protein: - 0.003
Refractometer
Required Volume:
Calibrators:
Refractometer
Required Volume: 1-2 drops
Calibrators:
DW - 1.000
5% NaCl - 1.022
9% Sucrose - 1.034
Principle of Refractometry
Compares the velocity of light in the AIR and velocity of light in the SOLUTION