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Clinical Microscopy > CSF > Flashcards

CSF Flashcards

1
Q

3rd major body fluid

A

CSF

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2
Q

CSF is produced by CHOROID PLEXUS by

selective filtration, at a rate of _____

A

20ml/hr

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3
Q

_______________ - where CSF flows

A

Subarachnoid space

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4
Q

Method of Collection

A

Lumbar Puncture

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5
Q

Lumbar puncture Sites

Common:
Adult:
Children

A

Lumbar puncture Sites

Common: L3, L4, L5; Common - L4
Adult: L3, L4
Children: L4, L5

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6
Q

CSF Order of Collection

Tube 1:
Tube 2:
Tube 3:
Tube 4:

A

CSF Order of Collection

Tube 1: Chem/Sero
Tube 2: Microbio
Tube 3: Hematology
Tube 4: Serology

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7
Q

CSF Tubes Temperature Requirement

Tube 1:
Tube 2:
Tube 3:

A

CSF Tubes Temperature Requirement

Tube 1: Frozen - -15C to -30C
Tube 2: Room Temperature - 19C to 22C
Tube 3: Refrigerator - 2C to 8C

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8
Q

Order in 1 CSF Tube ONLY

A

MicroBio > Hema > Chemistry

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9
Q

Normal CSF Appearance

A

Crystal Clear

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10
Q

CSF Appearance

Normal: 
Xanthochromia
 Pink
 Yellow
 Orange
 Yellow Green
 Brown
Bloody:
A

CSF Appearance

Normal: Crystal Clear
Xanthochromia
 Pink - Slight amount of OHb
 Yellow - Bilirubin
 Orange - Heavy hemolysis
 Yellow Green - Biliverdin
 Brown - Meningeal Metastatic Melanoma
Bloody: Traumatic Tap or Intracranial Hemorrhage
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11
Q

Traumatic Tap

Distribution of Blood:
Clot formation:
Supernatant:
Xanthochromia:
D-dimer:
A

Traumatic Tap

Distribution of Blood: Uneven - 1 > 2 > 3
Clot formation: Positive
Supernatant: Clear
Xanthochromia: Negative
D-dimer: Negative
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12
Q

Intracranial Hemorrhage

Distribution of Blood:
Clot formation:
Supernatant:
Xanthochromia:
D-dimer:
A

Intracranial Hemorrhage

Distribution of Blood: Even - 1 = 2 = 3
Clot formation: Negative
Supernatant: Xanthocromic
Xanthochromia:Positive
D-dimer: Positive
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13
Q

Present in Clotted Pellicle

A

Proteins and Clotting Factors

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14
Q

Cells in CSF

Normal:
Bacterial Meningitis:
Bone Marrow contamination:
RBCs in CSF:

A

Cells in CSF

Normal: Lymphocytes
Bacterial Meningitis: Neutrophils
Bone Marrow contamination: NRBCs
RBCs in CSF: Macrophages

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15
Q

CSF Dilutions

Clear
Slightly Hazy
Hazy
Slightly Cloudy
*Cloudy/Slightly Bloody
Bloody/Turbid
A

CSF Dilutions

Clear: UNDILUTED 1:1
Slightly Hazy - 1:10
Hazy - 1:20
Slightly Cloudy - 1:100
*Cloudy/Slightly Bloody - 1:200
Bloody/Turbid - 1:10,000
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16
Q

Cloudy CSF

A

1:200

17
Q

Predominant Cells in CSF

70% - ________
30% - ________

A

Predominant Cells in CSF

70% - LYMPHOcytes
30% - MONOcytes

18
Q

PLEOCYTOSIS

A

INCREASD number of NORMAL cells in the CSF

19
Q

CSF Proteins

§ MAJOR CSF Protein: _____________
§ 2nd most prevalent: _______________
§ Protein ONLY found in CSF: _________

A

CSF Proteins

§ MAJOR CSF Protein: Albumin
§ 2nd most prevalent: Transthyretin
§ Protein ONLY found in CSF: B2 Globulin - Transferrin

20
Q

ONLY protein found in CSF

A

B2 Globulin: Transferrin

21
Q

CHO-deficient transferrin

A

TAU Protein

22
Q

Proteins NOT Normally found in CSF

A

Fibrinogen
IgM
B-lipoprotein

23
Q

Predominant Cell Type - Bacterial Meningitis

A

Neutrophils

24
Q

CSF Glucose and Lactate is ______ in Viral Meningitis

A

NORMAL

25
Q

CSF Glucose is DECREASED in:
1.
2.
3.

A

CSF Glucose is decreased in:

  1. Bacterial
  2. Fungal
  3. Tubercular
26
Q

CSF Lactate is INCREASED in:
1.
2.
3.

A

CSF Lactate is INCREASED in:

  1. Bacterial
  2. Fungal
  3. Tubercular
27
Q

Predominant Cell in Bacterial Meningitis

A

Neutrophils

28
Q

Predominant Cell in Viral Meningitis

A

Lymphocytes

29
Q

In Fungal and Tubercular Meningitis, ___________ and ___________ are the predominant cell type.

A

Lymphocyte

Monocyte

30
Q

CSF - web-like clot or pellicle is formed

A

Tubercular (M. tuberculosis) Meningitis

31
Q

Findings:
(+) India ink preparations and microbial antigen tests
(+) Classic Starbust Pattern

A

Fungal Meningitis - C. neoformans

32
Q

Findings:

(+) Limulus Lysate test
(+) Culture
(+) Gram Stain - Gram Negative

A

Bacterial Meningitis

33
Q

Indirect Measure of Ammonia and used in the Diagnosis of Reyes Syndrome

A

CSF Glutamine

Clinical Microscopy (15 decks)

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