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Clinical Microscopy > Semen Analysis > Flashcards

Semen Analysis Flashcards

1
Q

Site of Sperm Maturation

A

Epidydymis

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2
Q

Source of High Zinc, ACP, and Proteolytic Enzyme

A

Prostate

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3
Q

Secretes Fructose

A

Seminal Vesicles

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4
Q

Produces Alkaline Urine

A

Bulbourethral Gland

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5
Q

Specimen Collection

Abstinence:
Preferred Method:
Collected:

A

Specimen Collection

Abstinence: 2-3 days, not > 5 days
Preferred Method: Masturbation
Collected: ENTIRE EJACULATE

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6
Q

In collecting seminal fluid, _______________ should be collected

A

ENTIRE EJACULATE

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7
Q

Semen analysis should be done only after ____________

A

Liquefaction

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8
Q

If not processed immediately, specimen should be kept at ______

A

37C

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9
Q

Absence of sperm cells

A

Azoospermia

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10
Q

No Ejaculate

A

Aspermia

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11
Q

Appearance

Normal:
Infection:
Red/Brown:
Yellow:

A

Appearance

Normal: Graysih white, tranluscent
Infection: Increased white turbidity
Red/Brown: Presence of Blood
Yellow: Prolonged Abstinence

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12
Q

Color - Prolonged Abstinence

A

Yellow

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13
Q

Yellow discoloration is due to __________________

A

INCREASED FLAVIN

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14
Q

Grading - Watery

A

0

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15
Q

Grading - Gel like

A

4

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16
Q

Normal Semen Volume

A

2-5 mL

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17
Q

Normal Semen

Liquefaction Time:
Color:
Viscosity:
Odor:
pH:
Volume:
Motility:
A

Normal Semen

Liquefaction Time: 30-60 minutes
Color: Grayish white
Viscosity: pour in droplets
Odor: Clorox-like
pH: 7.2 - 8.0
Volume: 2-5 ml
Motility: Grade 2
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18
Q

pH

A

7.2 - 8.0

19
Q

Effect of Increased prostatic fluid

A

DECREASED pH

20
Q

Effect of Prolonged Abstinence
1.
2.
3.

A

Increase Volume
Decrease Motility
Yellowish Color

21
Q

Specimen should be delivered to the lab within ____ of collection @Room Temp

A

1 hr

22
Q

Result if part of the FIRST portion is Missing

Increased:
Decreased:
Specimen will not ______.

A

Result if part of the FIRST portion is Missing

Increased: pH
Decreased: Sperm Count
Specimen will not LIQUEFY.

23
Q

Result if part of the LAST portion is Missing

Increased:
Decreased:
Specimen will not ______.

A

Result if part of the LAST portion is Missing

Increased: Sperm count
Decreased: Volume, pH
Specimen will not CLOT

24
Q

Sperm Motility Grading

4 - Rapid, \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
3 - \_\_\_\_\_\_\_\_\_, Lateral Movement
2 - \_\_\_\_\_\_\_\_\_, \_\_\_\_\_\_\_\_\_\_\_
1 -  \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
0 - \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
A

Sperm Motility Grading

4 - Rapid, Straight Line
3 - Slower, Lateral Movement
2 - Slow Forward, Noticeable Lateral Movement
1 - No Forward Progression
0 - No movement at all
25
Q

Used Counting Chamber for UNDILUTED SPECIMEN

A

Makler Counting Chamber

26
Q

Diluting Fluid in Specimen Analysis

A

NSS or Cold Tap Water

27
Q

NV - Sperm Motility

A

> 50% within 1 hr

28
Q

In sperm morphology, evaluate at least ______ sperm cells

A

200 sperm cells

29
Q

Sperm Morphology

Routine Criteria:
Strict Criteria (Kruger's):
A

Sperm Morphology

Routine Criteria: >30% normal morphology
Strict Criteria (Kruger's): >14% normal morphology
30
Q

Stain of Choice for Evaluating Sperm Morphology

A

Papanicolaou’s

31
Q

Immature Sperm Cell

A

Spermatid

32
Q

Most common cause of Male Infertility

A

Varicocele

33
Q

Seminal Fluid FRUCTOSE is tested w/in ____ hours or _________ to prevent fructolysis

A

2 hours

Frozen

34
Q

Screening Test for Fructose

A

Resorcinol Test

35
Q

Positive Result - Resorcinol Test

A

Orange Red Color

36
Q

Test for Sperm Viability

A

Modified Bloom’s Test

37
Q

Modified Bloom’s Test

Reagent: ________________
(+) Living Sperm: ____________

A

Modified Bloom’s Test

Reagent: Eosin-Nigrosine Stain
(+) Living Sperm: Unstained, Bluish white

38
Q

Test for Choline

A

Florence Test

39
Q

Florence test (nonspecific)

Tests for: _________
Rgt: __________
Result: (+) ______________

A

Florence test (nonspecific)

Tests for: CHOLINE
Rgt: Iodine crystals + KI
Result: (+) Dark brown rhombic crystals

40
Q

Positive Result - Florence Test

A

(+) Dark brown rhombic crystals

41
Q

Test for Spermine

A

Barbiero’s test

42
Q

Barbiero’s test (specific)

Tests for: _________
Rgt: __________
Result: (+) ______________

A

Barbiero’s test (specific)

Tests for: SPERMINE
Rgt: Sat. Picric Acid +TCA
Result: (+) Yellow leaf-like crystals

43
Q

Positive Result - Barbiero’s test

A

(+) Yellow leaf-like crystals

Clinical Microscopy (15 decks)

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