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Clinical Microscopy > Semen Analysis > Flashcards

Semen Analysis Flashcards

1
Q

Site of Sperm Maturation

A

Epidydymis

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2
Q

Source of High Zinc, ACP, and Proteolytic Enzyme

A

Prostate

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3
Q

Secretes Fructose

A

Seminal Vesicles

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4
Q

Produces Alkaline Urine

A

Bulbourethral Gland

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5
Q

Specimen Collection

Abstinence:
Preferred Method:
Collected:

A

Specimen Collection

Abstinence: 2-3 days, not > 5 days
Preferred Method: Masturbation
Collected: ENTIRE EJACULATE

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6
Q

In collecting seminal fluid, _______________ should be collected

A

ENTIRE EJACULATE

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7
Q

Semen analysis should be done only after ____________

A

Liquefaction

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8
Q

If not processed immediately, specimen should be kept at ______

A

37C

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9
Q

Absence of sperm cells

A

Azoospermia

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10
Q

No Ejaculate

A

Aspermia

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11
Q

Appearance

Normal:
Infection:
Red/Brown:
Yellow:

A

Appearance

Normal: Graysih white, tranluscent
Infection: Increased white turbidity
Red/Brown: Presence of Blood
Yellow: Prolonged Abstinence

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12
Q

Color - Prolonged Abstinence

A

Yellow

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13
Q

Yellow discoloration is due to __________________

A

INCREASED FLAVIN

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14
Q

Grading - Watery

A

0

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15
Q

Grading - Gel like

A

4

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16
Q

Normal Semen Volume

A

2-5 mL

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17
Q

Normal Semen

Liquefaction Time:
Color:
Viscosity:
Odor:
pH:
Volume:
Motility:
A

Normal Semen

Liquefaction Time: 30-60 minutes
Color: Grayish white
Viscosity: pour in droplets
Odor: Clorox-like
pH: 7.2 - 8.0
Volume: 2-5 ml
Motility: Grade 2
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18
Q

pH

19
Q

Effect of Increased prostatic fluid

A

DECREASED pH

20
Q

Effect of Prolonged Abstinence
1.
2.
3.

A

Increase Volume
Decrease Motility
Yellowish Color

21
Q

Specimen should be delivered to the lab within ____ of collection @Room Temp

22
Q

Result if part of the FIRST portion is Missing

Increased:
Decreased:
Specimen will not ______.

A

Result if part of the FIRST portion is Missing

Increased: pH
Decreased: Sperm Count
Specimen will not LIQUEFY.

23
Q

Result if part of the LAST portion is Missing

Increased:
Decreased:
Specimen will not ______.

A

Result if part of the LAST portion is Missing

Increased: Sperm count
Decreased: Volume, pH
Specimen will not CLOT

24
Q

Sperm Motility Grading

4 - Rapid, \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
3 - \_\_\_\_\_\_\_\_\_, Lateral Movement
2 - \_\_\_\_\_\_\_\_\_, \_\_\_\_\_\_\_\_\_\_\_
1 -  \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
0 - \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
A

Sperm Motility Grading

4 - Rapid, Straight Line
3 - Slower, Lateral Movement
2 - Slow Forward, Noticeable Lateral Movement
1 - No Forward Progression
0 - No movement at all
25
Used Counting Chamber for UNDILUTED SPECIMEN
Makler Counting Chamber
26
Diluting Fluid in Specimen Analysis
NSS or Cold Tap Water
27
NV - Sperm Motility
>50% within 1 hr
28
In sperm morphology, evaluate at least ______ sperm cells
200 sperm cells
29
Sperm Morphology ``` Routine Criteria: Strict Criteria (Kruger's): ```
Sperm Morphology ``` Routine Criteria: >30% normal morphology Strict Criteria (Kruger's): >14% normal morphology ```
30
Stain of Choice for Evaluating Sperm Morphology
Papanicolaou's
31
Immature Sperm Cell
Spermatid
32
Most common cause of Male Infertility
Varicocele
33
Seminal Fluid FRUCTOSE is tested w/in ____ hours or _________ to prevent fructolysis
2 hours | Frozen
34
Screening Test for Fructose
Resorcinol Test
35
Positive Result - Resorcinol Test
Orange Red Color
36
Test for Sperm Viability
Modified Bloom's Test
37
Modified Bloom's Test Reagent: ________________ (+) Living Sperm: ____________
Modified Bloom's Test Reagent: Eosin-Nigrosine Stain (+) Living Sperm: Unstained, Bluish white
38
Test for Choline
Florence Test
39
Florence test (nonspecific) Tests for: _________ Rgt: __________ Result: (+) ______________
Florence test (nonspecific) Tests for: CHOLINE Rgt: Iodine crystals + KI Result: (+) Dark brown rhombic crystals
40
Positive Result - Florence Test
(+) Dark brown rhombic crystals
41
Test for Spermine
Barbiero’s test
42
Barbiero’s test (specific) Tests for: _________ Rgt: __________ Result: (+) ______________
Barbiero’s test (specific) Tests for: SPERMINE Rgt: Sat. Picric Acid +TCA Result: (+) Yellow leaf-like crystals
43
Positive Result - Barbiero’s test
(+) Yellow leaf-like crystals

Clinical Microscopy (15 decks)

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