Unit II Beta oxidation

Question 1

Where in the cell does beta oxidation occur?

Answer 1

mitochondrial matrix

Question 2

What chaperone’s a FA to the mitochondrial surface?

Answer 2

fatty acid binding protein (cytoplasmic)

Question 3

When the FA first enters the cell, what is it activated by to form Acyl-CoA?

Answer 3

Acyl CoA synthetase

Question 4

Is the activation of a FA to Acyl-CoA an energy requirement step?

Answer 4

yes

Question 5

What is the location of a FA activated to Acyl-CoA?

Answer 5

outer mitochondrial membrane

Question 6

What type of fatty acid can pass directly to the mitochondrial matirx?

Answer 6

short chain fatty acids

Question 7

What transport system is used by LCFA’s and Acyl-CoA to cross over the inner mitochondrial membrane?

Answer 7

the Carnitine transport sytem

Question 8

What is another name for the carnitine transport system?

Answer 8

Carnitine palmitoyltransferase complex (CPT)

Question 9

What is considered the rate limiting step of beta oxidation?

Answer 9

CPT

Question 10

What inhibits CPT?

Answer 10

Malonyl-CoA

Question 11

What is the main enzyme of CPT?

Answer 11

acylcarnitine translocase (carnitine acyltransferase I)

Question 12

Once Acyl-CoA attaches to CPT-I, what gets removed?

Answer 12

CoA

Question 13

What replaces CoA in CPT-I?

Answer 13

carnitine

Question 14

Will CPT-II replace carnitine with CoA to get a FA into the mitochondrial matrix?

Answer 14

yes

Question 15

What are the four steps of Beta Oxidation?

Answer 15

Oxidation, Hydration, Oxidation, and Thiolysis (OHOT)

Question 16

What two additionalenzymes are required for unstaturated fatty acid degradation?

Answer 16

a reductase and isomerase

Question 17

Isomerase will break down _______ numbered double bonds

Answer 17

odd

Question 18

Reductase and isomerase will break down _______ numbered bonds

Answer 18

even

Question 19

What cofactor couples with reductase in unsaturated fatty acid degradation?

Answer 19

NADPH

Question 20

Isomerase, in unsaturated fatty acid degradation, will convert cis-Enoyl CoA?

Answer 20

trans-Enoyl CoA

Question 21

What is the final product of unsaturated fatty acid degradation?

Answer 21

propionyl CoA

Question 22

What does propionyl CoA get converted to?

Answer 22

succinyl CoA