Unit 2 quiz questions Flashcards
Which will be part of the differential media and biochemical tests lab?
PEA
EMB
TSA
catalase
thioglycolate
MacConkey Agar
A Gram-positive cell will grow on this media while a Gram - bacteria will be poor or inhibited
Phenylethyl Alcohol (PEA)
pink/red colonies on MacConkey Agar
gram-negative, lactose-fermenting bacteria
no color change/yellow colonies on MacConkey agar
Gram-negative, non-lactose-fermenting bacteria
you might expect to see purple to black colonies with or without a green metallic sheen on this type of media
Eosin Methylene Blue Agar (EMB)
a cell that uses an organic carbon source and obtains energy from light would be called a?
photoheterotroph
a microbe that grows only at the bottom of a tube of thioglycollate medium is probably a(n)
obligate anaerobe
a microorganism grows in the lab on medium containing mineral salts but not organic compounds. The organism is likely to be a(n)
autotroph
a(n) —- organism require(s) oxygen for growth
obligate aerobic
MacConkey agar plates represent —- medium
both a differential and a selective
ted heats some food just to boiling, and stores some of it immediately in a container which he places in the refrigerator. A week later he takes the food out and finds it spoiled. THe microbes that spoiled the food are probably — organsims
thermoduric
the events of binary fission in order
- the DNA is replicated
- the cell doubles in size
- the cell forms a septum
- the cell separates from its offspring
the use of salt and sugar in preserving various types of foods is an application of which of the following concepts?
osmotic pressure
anabolic reactions may be characterized by?
forming larger molecules from smaller ones
Anaerobic bacteria may use ________ as the final electron acceptor during anaerobic respiration.
either nitrate and sulfate
Prokaryotes produce the majority of their ATP
on the cytoplasmic membrane
Reactions in which atoms are rearranged within a molecule are catalyzed by
isomerases
The molecule that an enzyme acts upon is known as its
substrate
true or false: As the concentration of substrate increases, the activity of the enzyme decreases.
False
The amount of energy required for a reaction to occur is its activation energy.
true
What is an organism that must obtain its carbon in an organic form
Heterotroph
What is an organism that uses inorganic CO2 as its carbon source
Autotrophs
What is it called when microbes use light to make sugars
Phototroph
What is it called when microbes get its energy from chemical compounds?
Chemotroph
Which is NOT an essential element?
Calcium
What form of endocytosis ingests liquids
Pinocytosis
Pino Grigio -wine- liquid
What form of endocytosis ingests whole cells
Phagocytosis
What type of symbiosis is it when organisms live in an obligatory but mutually beneficial relationship?
Mutualism
What type of symbiosis is it when the partner receives benefits, while its partner is neither harmed nor benefited?
Commensalism
What type of symbiosis is it when a host organisms provides the parasitic microbe with nutrients and a habitat, and the host suffers from relationships?
Parasitism
Do not require oxygen for metabolism, but use it when it is present?
Facultative anaerobes
Lack the metabolic enzyme systems for use in oxygen in respiration — oxygen not required
Obligate anaerobe
An organism that cannot grow without oxygen
Obligate aerobe
Do not utilize oxygen but can survive and grow to a limited extent in its presence
Aerotolerant anaerobes
a bacterial genome is typically what?
a single circular DNA molecule
DNA replication and RNA transcription have many features in common. Which of the following does NOT occur in both processes?
a requirement for a primer
the bacterial chromosome is found where
- found in the nucleoid
- is circular
which contributes to the packaging of bacterial chromosomes?
gyrase
which of the following is a characteristic shared DNA and RNA polymerases?
direction of polymerases
which of the following is correct about bacterial transcription?
a. A single RNA polymerase produces all RNA transcripts.
b. Rho protein is required for termination of transcription.
c. The mRNA is extensively modified before transcription.
d. It occurs in the periplasm.
e. Various sigma factors recognize different promoter sequences.
various sigma factors recognize different promoter sequences
which of the following is CORRECT about BOTH bacterial and eukaryotic DNA replication?
Replication proceeds bidirectionally from the origin of replication
what is true of bacterial plasmids
they are small circular DNA molecules that can replicate autonomously
genetic elements known as promoters are initiation points in the process of?
transcription
