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Microbiology > Unit 2 Practical > Flashcards

Unit 2 Practical Flashcards

1
Q

Skim Milk Agar

A

Substrate: Casein

Enzyme: Casease

Product: Amino Acids

Positive Appearance: Clearing

Positive Bacteria: Bacillus

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2
Q

Tributyrin Agar

A

Substrate: Triglyceride

Enzyme: Lipase

Product: Glycerol, Fatty Acids

Positive Appearance: Clearing

Positive Appearance:

Staphylococus aureus

Serratia marcescans

Enterococcus

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3
Q

Gelatin Agar

A

Substrate: Collagen

Enzyme: Gelatinase

Product: Amino Acids

Positive Appearance: Liquid

Positive Bacteria: Bacillus

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4
Q

Starch Agar

A

Substrate: Starch

Enzyme: Amylase

Product: Maltose

Reagent: Iodine

Positive Appearance: Purple w/ Iodine

                                 Iodine binds starch

                                 CLEARING is positive

Positive Bacteria: Bacillus

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5
Q

DNA Agar

A

Methyl Green as a pH indicator

Substrate- DNA

Enzyme- DNAase

Product- Nucleotides

Positive Appearance- Clearing

Positive Bacteria- Serratia

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6
Q

Phenol Red

A

Carbohydrate Broth

phenol red- pH indicator

durham tube to show gas

Substrate- Glucose, Lactose, Sucrose

no enzyme specified

Product- Acid Fermentation- Yellow

           Alkaline Fermentation- Red

           Gas- CO<sub>2</sub>or H<sub>2</sub> - Bubbles

Positive Bacterium:

 E. Coli- Yellow and Gas in Glucose and Lactose          

              Red and no Gas in Sucrose 

 Staph aureus and Enterococcus-   Yellow and no Gas in all

 Pseudomonas- Red and no Gas in all
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7
Q

Methyl Red

A

Substrate- Pyruvate

Enzyme- Not specified

Product- Acid Fermentation

Positive Appearance- Red

Positive Bacteria- E coli

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8
Q

Voge’s Proskaurer

A

Substrate- Pyruvate

enzyme not specified

Product- Acetoin

Reagent- Barritt’s A B and Creatine

Positive Appearance- Red across top

Positive Bacteria- Enterobacter

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9
Q

SIM test

A

Sulfate

Indole

Motility

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10
Q

Sulfite Test

A

Substrate- Peptone iron

enzyme not specified

Product- H2S

Positive Appearance- Black

Positive Bacteria- Salmonella

                          Proteus
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11
Q

Indole Test

A

Substrate- Tryptophan

enzyme not specified

Product- Indole

Reagent- Kovac’s

Positive Appearance- Red

Positive Bacteria- E. coli

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12
Q

Motility Test

A

No Substrate, Enzyme or Product

Positive appearance- cloudy around stab

Positive bacteria- E coli

                          Enterobacter
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13
Q

Simmon’s Citrate Test

A

bromthymol blue- ph indicator

Substrate- Sodium citrate

Enzyme not specified

Product- Ammonia

Positive Appearance- Blue (alkaline)

Positive Bacteria- Enterobacter

                          Klebsiella

                          Pseudomonas

                          Citrobacter
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14
Q

Nitrate reduction

A

Substrate- Nitrate

Enzyme- Nitrite and nitrite reductase

Product- Nitrite and Ammonia or N2

Positive Appearance-

  Nitrite- E coli- Red with Nitrate A and B

  Ammonia or N<sub>2-</sub> Pseudomonas NO COLOR

  No product- Enterococcus Red with zinc
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15
Q

Triple Sugar Iron

A

phenol red- pH indicator

Substrate- Glucose, Lactose, Sucrose

Enzyme not specified

  1. all yellow, cracks- uses all sugars, gas- E coli
  2. all yellow, no cracks- uses all sugars, no gas- Staph, Enterococcus
  3. yellow/red- uses only glucose, no H2S- Shigella
  4. yellow/red/black- only glucose yes H2S- Proteus
  5. all red- alkaline and no H2S- Pseudomonas
  6. red/black- alkaline, yes H2S- Salmonella
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16
Q

Urea Test

A

Substrate- Urea

Enzyme- Urease

Product- Ammonia, CO2

Positive Appearance- Pink (alkaline)

Positive Bacteria- Proteus

17
Q

Decarboxylation

A

Substrate- Lysine, Arginine, Ornithine

Enzyme- Decarboxylase

Product- Removes acid group from amino acids

3/8” mineral oil to create anaerobic environment

Positive Appearance- Purple

Enterobacter- Positive for Lysine

                    Positive for Ornithine

Klebsiella- Negative for Ornithine

18
Q

Deamination

A

Substrate- Phenylalanine

Enzyme- Deaminase

Product- Removes amino group

Reagent- Ferric chloride

Positive appearance- Green

Positive bacteria- Proteus

19
Q
A

Skim milk agar

20
Q
A

Gelatin agar

21
Q
A

Starch agar

22
Q
23
Q
A

Phenol Red Test

24
Q
A

Methyl Red

25
Q
A

Voges Proskauer Test

26
Q
27
Q
A

Simmons citrate

28
Q
A

Nitrate Reduction Test

29
Q
A

Triple Sugar Iron test

31
Q
A

Decarboxylation

32
Q
A

Deanimation test

Microbiology (27 decks)

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