UCB Flashcards
What do UCB stem cells do?
foetal circulation is rich in stem cells, shortly after birth they migrate to BM
What types of stem cells are contained within UCB?
Haematopoeitic stem cells and mesenchymal stem cells
What is good about UCB derived MSC?
unproven. may be good for muscoskeletal
What is good about UCB derived HSC?
Re-engineering an entire tissue
What do HLA(human leukocyte antigens) do?
determine immunological identity of a cell encoded by MSC genes
What comes of unrecognised HLAs?
immune rejection
In light of immune rejection of unrecognised HLAs, what is good about UCB-HSC?
more tolerant of HLA mis match, maybe the only source of allogenic HSCs available to patients with rare HLA types
GVHD?
Graft vs Host disease
stages of GVHD? 4
1) mild skin rash 2) moderate larger skin rash and mild liver/stomach/intestinal disorders 3)severe redness of skin and moderate liver stomach intestine probs 4)life threatening blistering, peeling skin and severe gut issues
Do UCB-MSC exist?
Yes but rare
How are UCB-MSC isolated?
UCB collected from placenta and anticoagulant added, gradient centrifuged to get mononuclear cells, washed counted plated recovered = UCB-MSC!
What do UCB-MSC differentiate into?
Mesoderm - bone/fat, cartilage, muscle and endothelial
In what ways has repair efficacy been demonstrated by UCB-MSC in vivo?
In the mesoderm, bone healing and migration of UCB-MSC into a critical size femoral defect after xenotransplantation, in skeletal muscle in regeneration of SCID mice and cardiac muscle bc UCB progenitors are attracted to infarcted myocardium and significantly reduce myocardial infarction size.
What are advantaged to UCB-MSC?
Easy to obtain, no pain to patient, easy to cryopreserve and recover after 5 years, immune privilege (easier to find HLA match) and multipotent
What are disadvantages to UCB-MSC?
Few UCB-MSC in original UCB sample and slow initial growth rate. Limited proliferative potential and requirements for optimal cell culture unclear.
